User Responsibility - 3M Petrifilm 6400 Instrucciones De Producto

User Responsibility

Users are responsible for familiarizing themselves with product instructions and information. Visit our website at
www.3M.com/foodsafety, or contact your local 3M representative or distributor for more information.
When selecting a test method, it is important to recognize that external factors such as sampling methods, testing
protocols, sample preparation, handling, and laboratory technique may influence results.
It is the user's responsibility in selecting any test method or product to evaluate a sufficient number of samples with the
appropriate matrices and microbial challenges to satisfy the user that the chosen test method meets the user's criteria.
It is also the user's responsibility to determine that any test methods and results meet its customers' and suppliers'
requirements.
As with any test method, results obtained from use of any 3M Food Safety product do not constitute a guarantee of the
quality of the matrices or processes tested.
Storage
Store unopened 3M Petrifilm AC Plate pouches refrigerated or frozen at temperatures lower than or equal to 8°C (46°F).
Just prior to use, allow unopened pouches to come to room temperature before opening (20-25°C / <60% RH). Return
unused 3M Petrifilm AC Plates to pouch. Seal by folding the end of the pouch over and applying adhesive tape. To prevent
exposure to moisture, do not refrigerate opened pouches. Store resealed pouches in a cool dry place for no longer
than four weeks. It is recommended that resealed pouches of 3M Petrifilm AC Plates be stored in a freezer (see below) if
the laboratory temperature exceeds 25°C (77°F) and/or the laboratory is located in a region where the relative humidity
exceeds 50% (with the exception of air-conditioned premises).
To store opened pouches in a freezer, place 3M Petrifilm AC Plates in a sealable container. To remove frozen 3M Petrifilm
AC Plates for use, open the container, remove the plates that are needed and immediately return remaining plates to the
freezer in the sealed container. 3M Petrifilm AC Plates should not be used past their expiration date. The freezer that is
used for open pouch storage must not have an automatic defrost cycle as this would repeatedly expose the plates to
moisture which can damage the plates.
Do not use 3M Petrifilm AC Plates that show discoloration. Expiration date and lot number are noted on each package of
3M Petrifilm AC Plates. The lot number is also noted on individual 3M Petrifilm AC Plates.
Disposal
After use, 3M Petrifilm AC Plates may contain microorganisms that may be a potential biohazard. Follow current industry
standards for disposal.
Instructions for Use
Follow all instructions carefully. Failure to do so may lead to inaccurate results.
Sample Preparation
1. Use appropriate sterile diluents:
Butterfields phosphate buffered dilution water
dipotassium hydrogen phosphate solution
See section "Specific Instructions for Validated Methods" for specific requirements.
Do not use diluents containing citrate, bisulfite or thiosulfate with 3M Petrifilm AC Plates; they can inhibit growth. If
citrate buffer is indicated in the standard procedure, substitute with one of the buffers listed above, warmed to 40-45°C
(104-113°F).
2. Blend or homogenize sample.
3. For optimal growth and recovery of microorganisms, adjust the pH of the sample suspension to 6.6 - 7.2. For acidic
products, adjust the pH with 1N NaOH. For alkaline products, adjust the pH with 1N HCl.
Plating
1. Place the 3M Petrifilm AC Plate on a flat, level surface.
2. Lift the top film and with the pipette perpendicular to the inoculation surface dispense 1 mL of sample suspension onto
the center of bottom film.
3. Drop the top film down onto the sample.
4. Place the 3M™ Petrifilm™ Spreader with the recessed side down on the center of the 3M Petrifilm AC Plate. Press
gently on the center of the 3M Petrifilm Spreader to distribute the sample evenly. Spread the inoculum over the entire
3M Petrifilm AC Plate growth area before the gel is formed. Do not slide the 3M Petrifilm Spreader across the film.
5. Remove the 3M Petrifilm Spreader and leave the 3M Petrifilm AC plate undisturbed for at least one minute to permit the
gel to form.
, 0.1% peptone water
4
, saline solution (0.85-0.90%), bisulfite-free letheen broth or distilled water.
5
2
EN
, peptone salt diluent , buffered peptone water
4 5
(English)
,
5