Abaxis VETSCAN VS2 Manual Del Usuario página 130

C.3 Warnings and Precautions
• The diluent container in the reagent rotor is automati-
cally opened when the analyzer drawer closes. A rotor
with an opened diluent container cannot be re-used.
Ensure that the sample or control has been placed into
the rotor before closing the drawer.
• The reagent rotors are plastic and can crack or chip if
dropped. Never use a dropped rotor, as it may spray
biohazardous material throughout the interior of the
analyzer.
• Reagent beads may contain acids or caustic sub-
stances. The operator does not come into contact with
the reagent beads when following the recommended
procedures. If the beads are handled (such as when
cleaning up after dropping and cracking a reagent
rotor), avoid touching (with skin), ingesting, or inhal-
ing the reagent beads.
• Reagent beads and diluent contain sodium azide,
which can react with lead and copper plumbing to
form highly explosive metal azides. Reagents do not
come into contact with lead and copper plumbing
when following recommended procedures. However,
if the reagents do come into contact with such plumb-
ing, flush with a large volume of water to prevent
azide buildup.
• Calcium reagent beads contain arsenazo III sodium, a
possible carcinogen. The operator does not come into
contact with the reagent beads when following the rec-
ommended procedures. If the operator does come into
contact with the beads, immediately flush eyes or skin
with copious amounts of water for 15 minutes. If
inhaled, remove to fresh air. If swallowed, wash out
mouth with water. Call a physician.
C.4 Storage
Store reagent rotors in their sealed pouches at 2–8
º
(36–46 F). To use reagent rotors, remove the rotors in
their sealed foil pouches from the refrigerator. Open the
pouch, and remove the rotor just prior to running the
test.
Make sure rotors do not remain at room temperature for
more than a total of 48 hours. Open the pouch and
remove the rotor just before running the test.
Do not expose rotors, in or out of the foil pouches, to
direct sunlight or to temperatures above 32
Rotors must be used within 20 minutes of opening the
pouches. Rotors in opened pouches cannot be returned to
the refrigerator for later use.
C-4
C.5 Indications of Reagent Rotor
Instability or Deterioration
• When stored as described above, all reagents con-
tained in the reagent rotor are stable until the expira-
tion date printed on the rotor pouch. Do not use a rotor
after the expiration date. The expiration date is also
encoded in the bar code printed on the bar code ring.
The analyzer displays an error if the reagents have
expired.
• A torn or otherwise damaged pouch may allow mois-
ture to reach the unused rotor and adversely affect
reagent performance. Do not use a rotor from a dam-
aged pouch.
C.6 Sample Collection and Preparation
• The minimum required sample size is ~90 L of hepa-
rinized whole blood, heparinized plasma, serum, or
serum control. The reagent rotor sample chamber can
contain up to 120 L of sample.
• Fill specimen collection tubes at least halfway to pre-
vent an excessive concentration of anti-coagulant in
the patient sample.
• Samples collected in a heparinized micropipette
should be dispensed into the reagent rotor immedi-
ately following collection.
• Use only lithium heparin (green top) evacuated speci-
men collection tubes for whole blood or plasma sam-
ples. Use no-additive (red top) evacuated specimen
collection tubes or serum separator tubes (red or red/
black top) for serum samples.
• Whole blood samples obtained by venipuncture must
be homogeneous before transferring a sample to the
reagent rotor. Gently invert the collection tube several
times just before sample transfer. Do not shake the
collection tube, or hemolysis can result.
º
C
• The test must be begun within 10 minutes of transfer
ring the sample into the reagent rotor.
• Whole blood venipuncture samples should be run
within 60 minutes of collection
tions decrease approximately 5–12 mg/dL in 1 hour in
uncentrifuged samples stored at room temperature
AST samples may be artificially high due to in-vitro
hemolysis in stored whole blood
separated into plasma or serum and stored in capped
° °
C (90 F).
1. National Committee for Clinical Laboratory Standards
(NCCLS). 1984. Procedures for the Handling and Process-
ing of Blood Specimens; Tentative Standard. NCCLS docu-
ment H18-T. Villanova, PA: NCCLS; p. 219.
2. Overfield, CV, J Savory, and MG Heintges. 1972. Glycoly-
sis: a re-evaluation of the effect on blood glucose. Clin
Chim Acta 39: 35-40.
Methods Performance
1
. Glucose concentra-
2
.
3
. The sample can be