3M Attest 490M Manual Del Usuario página 7

INSTRUCTIONS FOR USE – Readout
Procedure
Instructions for Biological Indicator (BI) Activation
and Incubation
CAUTION: Allow steam-processed 3M™ Attest™ Biological Indicators to
cool for the recommended time period before activating� Activating or excessive
handling of the biological indicator before cooling may cause the glass ampoule
to burst�
CAUTION: Wear safety glasses when activating 3M™ Attest™ Super
Rapid Biological Indicators�
CAUTION: Wear safety glasses and gloves when activating the
3M™ Attest™ Rapid Readout Biological Indicator 1295�
WARNING: To reduce the risk associated with incorrect results
Do not incubate a 3M™ Attest™ Biological Indicator if, after processing and
before BI activation, it is observed to have a broken media ampoule� Retest the
sterilizer with a new biological indicator�
Activate and incubate 3M™ Attest™ Rapid Readout Biological Indicators
1295 within 1 hour of the completion of the sterilization cycle�
Allow steam-processed 3M™ Attest™ Biological Indicators to cool for the
recommended time period before activating�
Do not re-incubate 3M™ Attest™ Biological Indicators for which the Auto-reader
has already determined a result�
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Follow this procedure for every 3M™ Attest™ Biological Indicator (BI) to be read:
While wearing safety glasses (and gloves for
the 1295 BI), PLACE BI in Attest™ Biological
Indicator Activator�
CLOSE and SQUEEZE Attest™ Biological
Indicator Activator to close BI cap and crush
media ampoule�
Remove BI, visually VERIFY the glass ampoule
(containing growth media) has been crushed,
and FLICK BI to distribute purple growth media
to the growth chamber at the bottom of the vial�
INSPECT to verify media has transferred to
growth chamber at the bottom of the BI vial�
Orient flat side of activated BI towards front
of 3M™ Attest™ Mini Auto-reader 490M and
PLACE the BI into any incubation well� Observe
3M™ Attest™ Mini Auto-reader 490M display
to confirm correct placement of the BI� If the
BI is correctly seated, the 3M™ Attest™ Mini
Auto-reader 490M will display ●●●, ●●,
●, and then remaining minutes of incubation
directly below the incubating BI
RECORD BI result�
WARNING: Do not remove the biological indicator from the incubation
well until the (+) or (-) symbol on the display indicates the test is complete�