Magnetic Card Mode; Guide To Procedures - Binding Site Minineph Plus Manual De Usuario

MAGNETIC CARD MODE

This mode employs reagents that have been optimised for use on the MININEPH
Each vial of antibody is supplied with a swipe card containing all the information required to
convert light scatter units into protein concentration values. The information is kit lot specific
and must be loaded into the MININEPH
Parameters are loaded by passing the swipe card through the swipe card reader slot, in a
straight line from left to right across the front of the analyser with the magnetic strip facing
upward.
The timing of the assay is started automatically when the reagents are added to the cuvette.
This maintains the precision of the assay by removing any user variation in the timing of the
assay.
Loaded parameters are stored in the memory for future use so that if the same lot of
reagent is used again, the parameters do not need to be re-entered. If a different lot of
reagent is used, the previous parameters are overwritten when new details are entered from
the swipe card.
Assays should only be performed after referring to the methodology contained within the
MININEPH
PLUS
ANTIGEN EXCESS (Freelite only)
In Magnetic Card mode the MININEPH
and compares the results with reaction limits set through testing of an extensive sample
library. An intermediate scatter reading is taken after 1/3 of the assay time has elapsed. A
second intermediate reading is taken with1/3 of the assay time remaining. At the end of the
assay the change in scatter in the first third of the assay time is compared with the change
in scatter in the final third of the assay time and used to identify samples suspected of being
in antigen excess. Such samples are flagged with "XS" in the results and should be
remeasured at a higher sample dilution.
In UPC mode, the first and second intermediate readings are included in the results printout
and are identified as "XS1= " and "XS2= " respectively.
USER PREPARED CHEMISTRY (UPC) MODE
UPC mode is designed to allow the user to develop „in-house‟ assays. The assay
parameters are entered manually using the UPC program. Results are produced as scatter
units from which curves need to be generated. Refer to Appendix 2, UPC assay
development procedure, for guidance.

4.4.1 GUIDE TO PROCEDURES

This section shows the schematics used to explain the procedures in the following Set-up,
Magnetic card and UPC modes.
1. MININEPH
PLUS
The boxes show the screen output at the various stages of the program.
Insert Code: EIN023
assay package insert.
LCD Display Box
before using any new batch of reagent.
PLUS
monitors the reaction kinetics of each sample
PLUS
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PLUS
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Version: 11 August 2011