Waters HPLC Diagnóstico Y Corrección De Anomalías página 86

•Changes in Retention/Resolution Method Development
How Columns are Manufactured
•
1) A "BATCH"
• First Silica then Bonding
(Determines retention/selectivity/resolution)
Approve Approve
• • 2) 2)
Pack Columns,
• 3)
• Note: A BATCH* may be used to make many different
LOT #'s, and many thousands of columns
Approve and Ship
• 4)
•*
Note: Some column manufacturers buy packings and only pack columns
•They may have little if any control over chromatographic performance.
of Packing Material (particle) is prepared
"Chromatography" "Chromatography"
LOT # /
LOT # (Ligand/Endcapping)
Test for efficiency/back pressure
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