Waters HPLC Diagnóstico Y Corrección De Anomalías página 65

•Mobile Phase Composition is Critical for Reproducibility
•Clearly specify in your method
•Options A and A'
•600 mL
•6?? mL
• • 1000 mL 1000 mL
•Options A and A': Adding organic/aqueous directly to measuring vessel
• Need to specify which solvent is added first: e.g. MeOH to H
•Options B/C: Separate volumetric measurement or
•
•
•Standardize pH Adjustment – ie; BEFORE or AFTER organic is added
HOW the Mobile Phase is to be prepared
•60/40 (MeOH/Water)
•4?? mL
•400 mL
•Difference due to
•partial volumes*
•Note: Partial Volumes on mixing may give
• more or less than initial volumes
weighing is most accurate
•Options B/C
• • 600 mL 600 mL
• • • • abc gr abc gr abc gr abc gr
(not recommended)
O or H
2
• • 400 mL 400 mL
• • • • xyz gr xyz gr xyz gr xyz gr
•*
• • <1000 mL <1000 mL
•* 960 mL
O to MeOH
2
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