Waters HPLC Diagnóstico Y Corrección De Anomalías página 74

Proper Wetting of Bonded Chromatographic Proper Wetting of Bonded Chromatographic
Surface is Required for Retention Surface is Required for Retention
• Mobile
• Phase
What influence does this have on chromatography?
•Analyte
•The particles are very porous,
like the pores of a sponge –
99% of chromatographic
surface is inside the pores
•Mobile phase must •Mobile phase must
be allowed into the pore
•in order for chromatographic
•retention of the analyte
•to take place.
•If the pores are dry, the analyte
cannot get into the pores and it
will not be retained by the
chromatographic surface.
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