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Re- - wetting a Stationary Phase
Re
wetting a Stationary Phase
Once De- - wetted:
Once De
wetted:
Use a mobile phase containing > 40 % methanol or
other polar organic solvent
Difficult to use pressure to force aqueous mobile
Difficult to use pressure to force aqueous mobile
phase back into pores
Typically not practical because column outlet is at atmospheric pressure
•Inlet
•2200 psi
•P
•Length
•Outlet
•1 atm (14.7 psi)
©2007 Waters Corporation
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Solución de problemas
