Waters HPLC Diagnóstico Y Corrección De Anomalías página 68

Reversed Reversed- - Phase Retention Phase Retention Behaviour
Compounds Compounds
•> ± 2 pH units
retention (better reproducibility
35
at flat portions of curve)
30
25
20 20
15
10
•Ionized Base
5
0
0 1
Relative to Changes in pH Relative to Changes in pH
provides stable
•Range where
•pH control
•is critical
•pK
a
2 3 4 5
Behaviour of
•Non-ionized
Base
6 7 8 9
•±
2
of Basic Basic
10 11 12
•pH
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