Waters HPLC Diagnóstico Y Corrección De Anomalías página 123

Column Storage Column Storage
Store in Shipping Solvent for Longer Periods
of Time
— Flush with water/organic and then 100% organic
Column should be stored in solvent which
manufacturer recommends
For bonded phases, use organic solvent For bonded phases, use organic solvent
(eg. MeOH or ACN) Using non-aqueous
solvents minimizes hydrolysis.
Some bonded phases (CN) become unstable
in polar organic mobile phases.
Storage in water or buffer is then ok.
— Worst mobile phase for CN column is CH3CN
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