helena BioSciences SAS-1 Immunofix Instrucciones De Uso página 7

10. Following incubation, place a blotter comb into the holes of the antiserum template. Allow 2
minutes for the excess antisera to be absorbed, then remove the blotter combs and the template
from the gel surface.
11. i i ) ) S S A A S S - - 1 1 u u s s e e r r s s : : Remove the gel blocks using the Gel Block Remover and wash the gel in wash
solution for 5 minutes.
i i i i ) ) S S A A S S - - 1 1 P P l l u u s s a a n n d d S S A A S S - - 3 3 u u s s e e r r s s : : Place a blotter D (smooth side down) onto the surface of the gel,
leave for 10 seconds and remove.
12. i i ) ) S S A A S S - - 1 1 u u s s e e r r s s : : Place the gel on a blotter D agarose side up and place a blotter B (wetted in wash
solution) onto the surface of the gel followed by two blotter X's. Press the gel using the
Development Weight for 10 minutes.
i i i i ) ) S S A A S S - - 1 1 P P l l u u s s a a n n d d S S A A S S - - 3 3 u u s s e e r r s s : : Place a blotter D (smooth side down) onto the surface of the gel
and replace the antiserum template to hold the blotter flat. Blot the gel.
13. i i ) ) S S A A S S - - 1 1 u u s s e e r r s s : : Remove the blotters and place the gel in wash solution for 4 minutes with gentle
agitation.
i i i i ) ) S S A A S S - - 1 1 P P l l u u s s a a n n d d S S A A S S - - 3 3 u u s s e e r r s s : : Remove the blotter D.
14. i i ) ) S S A A S S - - 1 1 u u s s e e r r s s : : Remove the gel from the wash solution and place on a blotter D agarose side up.
Place a blotter B (wetted in wash solution) onto the surface of the gel followed by a blotter D.
Press the gel for 3 minutes.
i i i i ) ) S S A A S S - - 1 1 P P l l u u s s a a n n d d S S A A S S - - 3 3 u u s s e e r r s s : : Remove the gel blocks using the Gel Block Remover. Go to Step
16.
15. i i ) ) S S A A S S - - 1 1 u u s s e e r r s s : : Remove the blotters.
NOTE: Immediately after use, clean the antisera template with a mild, biocidal detergent.
If possible, scrub the bottom of the template with a toothbrush or small test tube brush. Do not
let the antisera dry on the template. Build up of antisera on the surface of the template will result
in the formation of bubbles during the antisera application step. Dry the antisera template
thoroughly. Water left in the holes will hinder the application of antisera for the next use. Store
the template upside down to increase the air circulation and thus the drying potential of the
applicator.
16. Attach the gel to the staining chamber holder.
17. Select the IFE test program on the staining unit and, following the prompts, Wash, Stain, Destain
and Dry the gel.
a) SAS-2 (Auto-Stainer)
Step Solution
Dry —-
Wash Wash solution
Stain Acid Violet Stain
Destain Destain solution
Dry —-
Wash Wash solution
Wash Wash solution
Dry —-
b) SAS-4 (Auto-Stainer)
Step
Wash
Wash
Time (mm:ss)
10:00
07:00
03:00
02:00
05:00
03:00
03:00
05:00
Time (mm:ss)
00:03
10:00
Port
4
5
2
4
4
Temp (° ° C C )
5
SAS-1 IMMUNOFIX
Temp (°C)
55
65
65
Other
Recirculate ON
Recirculate ON
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