helena BioSciences SAS-1 Immunofix Instrucciones De Uso página 5

ITEMS REQUIRED BUT NOT PROVIDED
Cat. No. 210200 Sample Applicator Blades (1 x 10)
Cat. No. 210300 Sample Applicator Blades (5 x 10)
Cat. No. 210100 Disposable sample cups (100)
Cat. No. 5014 Development Weight (2.3kg)
Cat. No. 3100 REP Prep
Drying oven with forced air capable of 60...70°C.
Saline solution (0.85% NaCl)
The following items are not required for standard serum IFE but may be required for further
investigation and Urine IFE investigation:
Cat. No. 220100 Antiserum to Human Urine Total Protein (2ml)
Cat. No. 220200 Antiserum to Human Urine Micro Proteins (2ml)
Cat. No. 220300 Antiserum to Human Urine Macro Proteins (2ml)
Cat. No. 220400 Antiserum to Human GAM Proteins (2ml)
Cat. No. 220700 Antiserum to Human Free & Bound Kappa Light Chain (2ml)
Cat. No. 220800 Antiserum to Human Free & Bound Lambda Light Chain (2ml)
Cat. No. 220500 Antiserum to Human Free Kappa Light Chain (2ml)
Cat. No. 220600 Antiserum to Human Free Lambda Light Chain (2ml)
Cat. No. 220900 Antiserum to Human Urine Pentavalent/Albumin (2ml)
Cat. No. 221000 Antiserum to Human Urine Pentavalent (2ml)
Cat. No. 9249 Antiserum to Human IgD
Cat. No. 9250 Antiserum to Human IgE
Cat. No. 9400 IFE Control Kit
SAMPLE COLLECTION AND PREPARATION
Freshly collected serum is the specimen of choice. Samples can be stored at 15...30°C for up to 4 days,
2...6°C for up to 2 weeks or 6 months at -20°C
Urine samples should be applied neat. Serum samples should be diluted according to the following
table using Sample Diluent:
Monoclonal Concentration
<3g/L
3-10g/L
10-25g/L
25-50g/L
STEP BY STEP PROCEDURE
1. Pipette 35µl of the sample into the appropriate well of the SAS-1 sample tray or disposable sample
cups.
i i ) ) S S A A S S - - 1 1 & & S S A A S S - - 1 1 P P l l u u s s u u s s e e r r s s : : Carefully place the sample tray onto the applicator drawer. Ensure that
the tray is pushed firmly down into position.
i i i i ) ) S S A A S S - - 3 3 u u s s e e r r s s : : Carefully locate the sample tray using the sample base locating pins. Ensure that the
tray is positioned securely.
2. Remove the gel from the packaging and:
i i ) ) S S A A S S - - 1 1 u u s s e e r r s s : : place the gel in the SAS-1, agarose side up, aligning the positive and negative sides
with the corresponding electrode posts.
6
.
SP Lane
1+2
1+2
1+2
1+2
3
SAS-1 IMMUNOFIX
κ κ
, , λ λ
G, A, M,
Neat
1+4
1+9
1+19
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