Brightfield Observation (Bf); Phase Contrast Observation (Ph) - Optika B-510 Serie Manual De Instrucciones

12.1 Brightfield Observation (BF)

1. Move the condenser slider all the way toward left to insert the
empty position. (Fig. 82)
2. Now repeat the steps described in the chapter "Brightfield
observation procedure".

12.2 Phase Contrast Observation (PH)

1. Center the condenser as already described in the chapter
10.9.
• This condenser does not have a swing-out lens, so the
operation described in step 2 is not necessary.
2. Move the condenser slider all the way toward right to insert
the phase ring dedicated to 40X objective. (Fig. 83)
3. Insert 40x objective into the light path.
4. Open aperture diaphragm.
5. Place a specimen on the stage and focus.
6. Remove one eyepiece and insert the centering telescope.
(Fig. 75)
7. Rotate the upper part of the centering telescope until the two
phase rings (one dark and one bright) visible in the telescope
are in focus. (Fig. 75-76)
8. Using centering screws on the slider ① (Fig. 84), center the
phase rings as already described in the chapter 11.3.
9. At the end remove the centering telescope, reinstall the
eyepiece and begin observation.
• With 40x objective it may be useful to slightly raise the
condenser, to obtain a better projection of the phase
rings. This is not a defect.
• With the 4X objective, the condenser could have a dark
halo at the periphery of the field of view. This is not to be
considered a defect.
10. For observation of asbestos fibres in phase contrast, remove
the provided 10X eyepieces and insert the 12.5X eyepieces.
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