Troubleshooting - VWR Visiscope 400 Serie Manual De Instrucciones

Troubleshooting

Review the information in the table below to troubleshoot operating problems.
PROBLEM
I. Optical Section:
1. The illumination is open, but
the field of view is dark.
2. The edge of the field of view
is vignetted or the brightness is
asymmetric.
3. Dust and stains can be seen in
the field of view.
4. There is an apparent double
image.
5. Poor image quality:
The image is not sharp
The contrast is not high
The details are not clear
The phase contrast is low.
CAUSE
The plug of the LED holder
is not connected to the il-
lumination set
The brightness is too low
Too many colour filters have
been stacked
The nosepiece is not in the
correct position
The color filter is partially
inserted
The phase contrast slider is
not in the proper position
There are stains and dust on
the specimen
There are stains and dust on
the eyepiece
The size of the aperture
diaphragm is too small
The nosepiece is not in the
center of the light path
The aperture diaphragm in
the view of field is opened
too much or too little
The lenses (condenser,
objective, eyepieces are
culture dish) is dirty
In phase contrast observa-
tion, the bottom thickness
of the sample is more than
1.2mm
A bright field objective is
used for phase contrast
observation
The condenser ring is not
aligned with the objective
phase ring
The light ring and/or the
phase contrast ring is not
centered
The objective used is not
compatible with the phase
ring
The phase contrast depends
on the sample position
14
SOLUTION
Connect them
Adjust to a proper setting
Minimize the number of the filters
Turn the nosepiece to a click stop
Insert the filter to full depth
Move the slider to a click stop
Clean the specimen
Clean the eyepiece
Open the aperture diaphragm
Turn the nosepiece to a click stop
Adjust the aperture diaphragm
Thoroughly clean all the optical system
Use a sample holder whose bottom
thickness is less than 1.2mm
Switch to a phase contrast objective
Adjust the condenser ring to match the
objective phase ring
Adjust the bolts to center them
Please use a compatible objective
The sample holder is not flat. Move the
sample around until a compatible area
is found.