1. Vertical PAGE may sometimes result in high currents. High current may cause
the build-up of heat which can affect sample migration and gel resolution as a
result. To counteract the adverse effects of heat generation, the manufacturer
recommends using an effective chiller unit, preset to 4°C.
2. For active temperature regulation attach two short lengths of hose from the inlet
and outlet ports of the chiller unit to the respective outlet and inlet connectors of
the cooling coil sitting within the shiroGEL VERTICAL 20 outer tank.
3. Ensure that the flow rate of the chiller unit is set at a mum 1L/minute. Do not
exceed this limit as damage to the cooling platform might occur.
4. If using a chiller unit which exceeds this limit, a T-connector may be used. The
T-connector can divert some coolant volume back to the recirculating chiller
reducing the pressure incident within the coil.
The unit is now ready to be used with active cooling.
Connecting to a Chiller Unit
26