Stock Solutions - VWR shiroGEL Vertical 20 Manual De Instrucciones

Stock Solutions for SDS PAGE gels:
Stock 30% Acrylamide Gel Solution:-
30.0 g acrylamide
0.8 g methylene bisacrylamide
Distilled Water to 100ml
Stock 4 X Resolving Gel Tris (1.5 M Tris
To 110ml Distilled Water add 36.4 g of Tris base
Add 8ml of 10 % SDS
Adjust pH to 8.8 with 1N HCl
Adjust the final volume to 200ml with Distilled Water.
Stock 4 X Stacking Tris (0.5 M Tris
To 110ml Distilled Water add 12.12 g of Tris base
Add 8ml of 10 % SDS
Adjust pH to 6.8 with 1N HCl
Add Distilled Water to a final volume of 200ml
Stock 4 X Tris-glycine tank buffer - SDS
36 g Tris base
172.8 g glycine
Distilled Water to 3 L
1 x Tris-glycine tank buffer - SDS
750ml of 4 X Tris-glycine reservoir buffer - SDS
30ml of 10 % SDS
Distilled Water to 3L
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HCl pH8.8, 0.4 % SDS)
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HCL pH6.8, 0.4 % SDS)
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