Simultaneous Observation In Transmitted Light + Fluorescence - Optika Italy SZR Serie Manual De Instrucciones

11. Simultaneous observation in Transmitted Light + Fluorescence

• This microscope allows observation in Transmitted Light in combination with Fluorescence. Samples with
rapid decay must first be observed in Fluorescence and then in Transmitted Light. The combined observation
allows you to easily identify some areas of the sample that emit fluorescence.
1. Turn on the microscope main switch.
2. Move the fluorescence filter selector to an empty position or, if the filter holder is full, to the position containing the UV
filter.
3. Turn the transmitted light intensity adjustment knob to adjust the brightness to the sample under observation.
4. Move the lever located on the right side of the base, to select Brightfield, OIC or Darkfield.
5. Focus the sample.
6. Move the fluorescence filter selector to the desired position.
7. Adjust the light intensity of the reflected light.
8. To obtain the proper observation of the sample, adjust the light intensity of the transmitted light to modulate the intensity
of the fluorescence with the one of the transmitted light (Brightfield, OIC or Darkfield).
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