Centrifuge Rotor Usage Guide - VWR 1814 Manual De Instrucciones

To Calculate Separation Acceleration & RPM:
RCF = 11.18 x r x (n/1000) 2
Where: r = radius in cm
n = speed in rpm
The effective centrifugal radius of the 18 x 1.5ml rotor is 7.3 cm; 24 x 1.5ml rotor is 8.1 cm
*Value given is for the maximum speed of the centrifuge, 13,300 rpm.

Centrifuge Rotor Usage Guide

The rotors are intended for the sole purpose of spinning samples in the microcentrifuge.
Use of this product in a manner not specified by the manufacturer may impair or negate
any safety protection provided by the maker.
Good laboratory practice includes observing and following the safety precautions listed in this
guide.
As with any centrifuge, always wear shatterproof, full coverage, wraparound eye
protection and laboratory clothing with long sleeves. Observe all appropriate safety
precautions for the materials in use as detailed by Material Safety Data Sheets.
Rotor Maintenance
Visually inspect the rotor for mechanical or chemical damage prior to each use. Do not use the
rotor if any damage or change is evident. Clean any spills immediately.
Routinely perform mechanical and chemical inspections as follows:
• Check the web area for cracks and crazing.
• Check the rotor underside, including hub area for cracks and crazing.
• Check the outer rim of the rotor.
- Chemical damage appears as discoloration, crazing, granulation, peeling or similar
deterioration of the finish.
- Hidden mechanical damage may be detected by an increase in noise or vibration during a spin. If noise level increases, stop use immediately.
If any such changes are apparent, discontinue use immediately.
Loading Samples
• Never spin samples which are flammable, explosive or which chemically interact vigorously
when mixed.
• Discard any rotor and rotor cover that becomes contaminated by radioactive samples, as
the exposure may reduce the strength of the rotor material.
RCF Quick Reference (approx.)
RPM 1814/16
1000 82
2000 326
3000 735
4000 1306
5000 2040
6000 2938
7000 3999
8000 5223
9000 6611
10000 8161
11000 9875
12000 11752
13000 13793
14000 15996
2416
91
362
815
1449
2264
3260
4437
5796
7335
9056
10958
13040
16019*