5. Reposition the slide and raise the condenser until
the oil on the lens of the condenser is in contact
with the slide.
6. Move the area to be observed at the center of the
optical path using a low magnification objective
(10x or 40x).
7. Focus the specimen.
8. Insert in the light path100X oil/Iris objective.
This pre-positions all system components in
preparation for adding oil. Move the immersion
objective to an adjacent position of the nosepiece
and apply the oil to the sample. (Fig. 27)
9. Actually, the situation must be in which the slide is
completely immersed in oil both in the lower part
(condenser-glass interface) ①, and in the upper
part (glass-lens interface) ②. (Fig. 28)
10. Remove one eyepiece and insert the centering
telescope in the empty eyepiece sleeve. (Fig. 29)
Page 20
Fig. 27
②
①
Fig. 28
Fig. 29