Operation; Observation Head; Place The Specimen On The Stage; Illumination System Settings - VWR VisiScope 384 Serie Manual De Instrucciones

Operation

Observation head

Loosen the lock-screw, turn the observation head to a comfortable position for observation, and then lock the
lock-screw.

Place the specimen on the stage

Lock the specimen slide on the mechanical stage using the slide holder. Ensure that the specimen is centred
over the stage opening by adjusting the coaxial stage controls.

Illumination system settings

The microscope is fitted with a white LED illuminator. Before turning on the illumination system, read the
section about electrical safety precautions. Insert the plug of the cable into the power socket and turn on the
switch on the back of the main body. Turn the brightness adjustment knob to a brightness suitable for obser-
vation.

Adjust interpupillary distance

Hold the right and left side of the observation head with both hands and adjust the interpupillary distance by
turning the two parts until one circle of light can be seen, when looking down the microscope with both eyes.
The white dot (°) placed on the right eyepiece shows the set interpupillary distance. Just remember this value
to help on later settings.

Focus tension adjustment

The tension of the coarse focusing knob is preset by factory. To change the tension according to your prefer-
ence, just rotate the knob clockwise in order to increase it.
Excessive tension could damage the mechanism of focus. A too loosed tension causes the descent of the
stage by gravity or a sudden loss of focus. In this case, rotate the knob to increase the tension.

Diopter adjustment

Turn the dioptric adjustment ring on the right eyepiece dioptric up to align the bottom with the graduated ring.
Turn the coarse focus knob in order to focus the slide with an objective with low magnification. Adjust the
fine focus knob until you obtain a clear and defined picture observing with the right eye, and then repeat the
operation with the left dioptric compensation ring and the left eye. When the image appears in focus, choose
the necessary objective with the revolving nosepiece.

Condenser

Raise or lower the condenser through the knob to obtain a clear and uniform illumination of the sample.
To center the condenser: completely close the iris diaphragm. Using the condenser centering screws, move
the diaphragm in the center of the field of view. Then gradually expand the diaphragm until it is tangent to the
edges of the field of view. If necessary, you can perform an additional adjustment.
The condenser is centered when the edges of iris diaphragm are tangent to the field of view.

Numerical aperture setting

The value of the numerical aperture (N.A.) of the diaphragm is an indication of the contrast of the illumination
system. Matching the value of illumination system's N.A. with that of the objective ensures the best results in
terms of contrast and image quality. To set the numerical aperture of the Illuminator, adjust the opening of the
iris diaphragm. In this way you control contrast and image resolution. For samples with low contrast set the
iris to about 75% of the value of the objective's numerical aperture.
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