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Idiomas disponibles
Idiomas disponibles
3) PHASE
CONTRAST
(PH)
1. Center
the
condenser
described at pag. 50.
2. Rotate the condeser turret to insert the
"10/20" position.
3. Insert 10x objective into the light path.
4. Open aperture diaphragm.
5. Place a specimen on the stage and focus.
6. Remove one eyepiece and insert the
centering telescope. (Fig.22)
7. Rotate the upper part of the centering
telescope until the two phase rings
(one dark and one bright) visible in the
telescope are in focus. (Fig. 23)
8. Using centering screws on the condenser
①, center the phase rings to make the
bright ring ② be concentric to the dark
ring ③.
9. Insert 20x objective (do not rotate the
condenser turret) and check the centering
of the two rings. (Fig. 25)
10. Repeate the same operation with other
objectives to check the ring centering:
40x objective – turret position "40", 100x
objective – turret position "100".
11. At
the
end
remove
telescope, reinstall the eyepiece and
begin observation.
•
With 40x and 100x objectives it may be
useful to slightly raise the condenser,
to obtain a better projection of the
phase rings. This is not a defect.
•
With the 4X objective, the condenser
could have a dark halo at the periphery
of the field of view. This is not to be
considered a defect.
Use of the green filter (Fig. 26)
•
The green filter is used to increase the
contrast of the image during phase
contrast observation.
•
Place the filter on the field lens of the
microscope (Fig. 26) and begin the
observation.
•
For observation in brightfield or darkfield
it is advisable to remove the filter from the
optical path.
OBSERVATION
as
already
the
centering
CONDENSER PHASE
RING (BRIGHT)
②
OBJECTIVE PHASE
③
RING (DARK)
Page 53
UPPER PART OF CEN-
TERING TELESCOPE
①
Fig.22
Fig.23
Fig.24
Fig.25
Fig.26
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