Rapid Coliform Count Plate - 3M Petrifilm 6402 Manual Del Usuario

(Great Britain / U.S.)
3M Petrifilm
™ ™
6402/6412

Rapid Coliform Count Plate

DESCRIPTION
The 3M™ Petrifilm™ Rapid Coliform Count (RCC) Plate is a sample-ready-culture-medium system which contains Violet Red Bile (VRB) nutrients,
a cold-water-soluble gelling agent, a pH indicator to detect acid and a tetrazolium indicator that facilitates colony enumeration. Petrifilm RCC
Plates are useful for the enumeration of coliform bacteria in the food and dairy industries. Petrifilm RCC Plate components are decontaminated
though not sterilized. 3M Microbiology is certified to ISO (International Standards Organization) 9001.
AOAC International and U.S. FDA Bacteriological Analytical Manual (BAM) define coliforms as gram-negative rods which produce acid and gas
from lactose during metabolic fermentation. As colonies grow on the Petrifilm RCC Plate and produce acid, the pH indicator in the plate changes
from red-orange to yellow, providing a presumptive indication of coliforms. Gas trapped around coliform colonies indicates confirmed coliforms.
ISO defines coliforms by their ability to grow in method-specific, selective media. ISO method 4832, enumerating coliforms by the colony count
technique, defines coliforms by colony size and acid production on VRB with lactose (VRBL) agar. On Petrifilm RCC Plates, these acid-producing
coliforms are indicated by yellow acid zones, or red colonies with or without gas. ISO method 4831, enumerating coliforms by the Most Probable
Number (MPN) method, defines coliforms by their ability to grow and produce gas from lactose in a selective broth. On Petrifilm RCC Plates these
coliforms are indicated by red colonies associated with gas. AFNOR has validated Petrifilm RCC Plates as a method in comparison to ISO method
4831 and ISO method 4832.
CAUTIONS
3M has not documented Petrifilm RCC Plates for use in industries other than food and beverage. For example, 3M has not documented Petrifilm
RCC Plates for testing water, pharmaceuticals or cosmetics. Acceptance of the Petrifilm RCC Plate method for the testing of water per an accepted
local government regulation is at the sole discretion and responsibility of the end-user.
Petrifilm RCC Plates have not been tested with all possible food products, food processes, testing protocols or with all possible strains of coliform
or other bacteria.
Petrifilm RCC plate do not differentiate any one coliform strain from another.
Do not use Petrifilm RCC Plates in the diagnosis of conditions in humans or animals.
For information on documentation of product performance contact your official 3M Microbiology products representative.
USER RESPONSIBILITY
No one culture medium will always recover the exact same strains or enumerate a particular strain exactly as does another medium. In addition,
external factors such as sampling methods, testing protocols, preparation time and ith high sugar content may increase the potential for gas
production from non-coliform Enterobacteriaceae.
It is the user's responsibility in selecting any test method to evaluate a sufficient number of samples with particular foods and microbial
challenges to satisfy the user that the chosen test method meets the user's criteria.
It is also the user's responsibility to determine that any test methods and results meet its customers' or suppliers' requirements.
As with any culture medium, Petrifilm RCC Plate results do not constitute a guarantee of quality of food or beverage products or processes that
are tested with the plates.
The user must train its personnel in proper testing techniques: for example, Good Laboratory Practices (U.S. Food and Drug Administration,
Title 21, Part 58 of the Cod e of Federal Regulations) or ISO 17025.
DISCLAIMER OF WARRANTIES / LIMITED REMEDY
UNLESS OTHERWISE PROHIBITED BY LAW, 3M DISCLAIMS ALL EXPRESS AND IMPLIED WARRANTIES, INCLUDING, BUT NOT LIMITED TO,
ANY WARRANTIES OF MERCHANTABILITY OR FITNESS FOR A PARTICULAR USE. If any 3M Petrifilm Plate is proven to be defective, 3M or its
authorized distributor will replace or, at its option, refund the purchase price of any plate. These are your exclusive remedies. You must promptly
notify 3M within sixty days of discovery of any suspected defect in a product and return the product to 3M. Please call Customer Service
(1-800-328-1671 in the U.S.) or your official 3M Microbiology products representative for a Returned Goods Authorization.
LIMITATION OF 3M LIABILITY
UNLESS OTHERWISE PROHIBITED BY LAW, 3M WILL NOT BE LIABLE TO USER OR OTHERS FOR ANY LOSS OR DAMAGE, WHETHER DIRECT,
INDIRECT, SPECIAL, INCIDENTAL OR CONSEQUENTIAL DAMAGES, INCLUDING, BUT NOT LIMITED TO, LOST PROFITS. Except where prohibited
by law, in no event shall 3M's liability under any legal theory exceed the purchase price of the plates alleged to be defective. Customer may
have additional rights and should seek advice in country of purchase.
STORAGE AND DISPOSAL
Store unopened Petrifilm Plate pouches refrigerated or frozen at temperatures less than or equal to 8ºC (46ºF). Just prior to use, allow unopened
pouches to come to room temperature before opening. Return unused plates to pouch. Seal by folding the end of the pouch over and taping shut.
To prevent exposure to moisture, do not refrigerate opened pouches. Store resealed pouches in a cool dry place for no longer than one month.
It is recommended that resealed pouches of Petrifilm Plates be stored in a freezer (see below) if the laboratory temperature exceeds 25ºC (77ºF)
and/or the laboratory is located in a region where the relative humidity exceeds 50% (with the exception of air-conditioned premises).
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