Idiomas disponibles
Idiomas disponibles
P P E E R R F F O O R R M M A A N N C C E E C C H H A A R R A A C C T T E E R R I I S S T T I I C C S S
a a ) ) R R e e p p r r o o d d u u c c i i b b i i l l i i t t y y
The SAS-MX Acid Hb gel is a qualitative system for the identification of haemoglobin bands.
Using a control material containing Hb's A, S and A
single gel and between different gels. No bands were missing and no additional bands were
observed between applications.
b b ) ) S S e e n n s s i i t t i i v v i i t t y y
0.08 g/dL per band, determined as the lowest concentration of haemoglobin which was evident as
a discrete band on the completed gel.
c c ) ) L L i i n n e e a a r r i i t t y y
4 g/dL per band, based upon the maximum concentration of haemoglobin per band which allows
separated bands to be satisfactorily resolved from each other without protein overloading.
This procedure is not intended for densitometeric scanning.
B B I I B B L L I I O O G G R R A A P P H H Y Y
1. Wintrobe, Maxwell M., Clinical Hematology, 6th Edition, Lea and Febiger, Philadelphia, 1967.
pages 145-167.
2. Fairbanks, V.F., 'The Nomenclature and Taxonomy of Hemoglobin Variants', Diagnostic Medicine,
Nov/Dec., 53-58, 1980.
3. Schneider, R.G., Hightower, B.J. and Barwick, R.C., 'Laboratory Identification of the
Hemoglobins', Lab Management, 1981; August: 29-43.
4. Center for Disease Control, Laboratory Methods for Detecting Hemoglobinopathies, U.S.
Department of Health and Human Services/Public Health Service, 1984.
5. Schneider, R.G.,' Methods for Detection of Hemoglobin Variants and Hemoglobinopathies in the
Routine Clinical Laboratory', CRC Critical Reviews in Clinical Laboratory Sciences, 1978.
6. Schneider, R.G., Hightower, B., Hosty, T.S., Ryder, H., Tomlin, G., Atkins, R., Brimhall, B., and
Jones, R.T.,'Abnormal Hemoglobins in a Quarter Million People', Blood, 1976; 48(5) : 629-637.
7. Huisman, T.H.J. and Schroeder, W.A., 'New Aspects of the Structure, Function and Synthesis of
Hemoglobins'. CRC Press, Cleveland, 1971.
8. Schmidt, R.M., Huisman, T.H.J.,and Lehmann, H., The Detection of Hemoglobinopathies. CRC
Press, Cleveland, 1974.
9. Weatherall, D.J. and Clegg, J.B., The Thalassemia Syndromes, Blackwell Scientific Publications,
Oxford, 1972.
10. Lehman, H. and Huntsman, R.G., Man's Haemoglobins, J.B. Lippincott Co., Philadelphia, 1974.
, the same band patterns were seen within a
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