Idiomas disponibles
Idiomas disponibles
SAS-MX ACID HB-10
I I N N T T E E N N D D E E D D P P U U R R P P O O S S E E
The SAS-MX Acid Hb-10 kit is intended for the separation of human haemoglobins by agarose gel
electrophoresis.
Haemoglobins (Hb) are a group of proteins whose chief functions are to transport oxygen from the
lungs to the tissues and carbon dioxide in the reverse direction. They are composed of polypeptide
chains called globin, and iron protoporphyrin haem groups. A specific sequence of amino acids
constitutes each of the four polypeptide chains. Each normal haemoglobin molecule contains one pair
of alpha and one pair of non-alpha chains. In normal adult haemoglobin (HbA), the non-alpha chains
are called beta. The non-alpha chains of foetal haemoglobin are called gamma. A minor (3%)
haemoglobin fraction called HbA
contains alpha and delta chains. Two other chains are formed in the
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embryo.
The major haemoglobin in the erythrocytes of the normal adult is HbA and there are small amounts of
HbA
and HbF. In addition, over 400 mutant haemoglobins are now known, some of which may cause
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serious clinical effects, especially in the homozygous state or in combination with another abnormal
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haemoglobin. Wintrobe
divides the abnormalities of haemoglobin synthesis into three groups.
1. Production of an abnormal protein molecule (e.g. sickle cell anaemia).
2. Reduction in the amount of normal protein synthesis (e.g. thalassaemia).
3. Developmental anomalies (e.g. hereditary persistence of foetal haemoglobin (HPFH)).
The two mutant haemoglobins most commonly seen are HbS and HbC. Hb Lepore, HbE, HbG-
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Philadelphia, HbD-Los Angeles, and HbO-Arab may be seen less frequently
.
Electrophoresis is generally considered the best method for separating and identifying
haemoglobinopathies. The protocol for haemoglobin electrophoresis involves step-wise use of two
3-8
systems
.
Initial electrophoresis is performed in alkaline buffers.
However, because of the
electrophoretic similarity of many structurally different haemoglobins, the evaluation must be
supplemented by acid buffer electrophoresis which measures a property other than electrical charge.
This method is based on the complex interactions of the haemoglobin with an acid electrophoretic
buffer and the agarose support. The SAS-MX Acid Hb-10 procedure is a simple procedure requiring
minute quantities of haemolysate to provide complementary evidence (along with the results from
SAS-MX Alk Hb-10 analysis) of the presence of HbS, HbC and HbF as well as several other abnormal
hemoglobins.
W W A A R R N N I I N N G G S S A A N N D D P P R R E E C C A A U U T T I I O O N N S S
All reagents are for in-vitro diagnostic use only. Do not ingest or pipette by mouth any kit component.
Wear gloves when handling all kit components. Refer to the product safety data sheet for risk and
safety phrases and disposal information.
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