helena BioSciences SAS-3 Immunofix Instrucciones De Uso página 4

3. Destain Solution
Dilute the contents of a bottle of Destain A to 2 litres of purified water. Then add the contents of
a bottle of Destain B and add a further 3 litres of purified water, slowly.
4. Wash Additive Solution
Contains concentrated Wash Additive Solution. Dilute the contents of the bottle to 5 litres with
saline solution.
5. Sample Diluent
Contains Tris / barbital buffer with bromophenol blue and sodium azide as preservative.
The diluent is ready for use as packaged.
6. SAS-3 IFE Antisera Kit
Contains SP protein fixative (containing acetic acid and sulphosalicylic acid), and monospecific
antisera to human immunoglobulins - IgG, IgA, IgM, Kappa Light Chain (free & bound) and Lambda
Light Chain (free & bound). All antisera contain sodium azide as preservative. The antisera are
ready for use as packaged.
7. Other Kit Components
Each kit contains Instructions For Use and sufficient Blotters C, D and Blotter Combs to complete
10 gels.
STORAGE AND SHELF-LIFE
1. SAS-3 IFE Gel
Gels should be stored at 15...30°C and are stable until the expiry date indicated on the package.
DO NOT REFRIGERATE OR FREEZE. Deterioration of the gel may be indicated by 1) crystalline
appearance indicating the gel has been frozen, 2) cracking and peeling indicating drying of the gel
or 3) visible contamination of the agarose from bacterial or fungal sources.
2. Acid Violet Stain
The stain concentrate should be stored at 15...30°C and is stable until the expiry date indicated on
the label. Diluted stain solution is stable for 6 months at 15...30°C. It is recommended to discard
used stain immediately to prevent depletion of staining capability. Poor staining performance may
indicate deterioration of the stain solution.
3. Destain Solution
The destain concentrate should be stored at 15...30°C and is stable until the expiry date indicated
on the label. Diluted destain solution is stable for 6 months at 15...30°C.
4. Wash Solution
The Wash Solution concentrate should be stored at 15...30°C and is stable until the expiry date
indicated on the label. The wash solution is stable for 6 months at 15...30°C.
5. Sample Diluent
The Sample Diluent should be stored at 15...30°C and is stable until the expiry date indicated on
the label. Cloudiness may indicate deterioration.
6. SAS-3 IFE Antisera Kit
The antisera kit should be stored at 2...6°C and is stable until the expiry date indicated on the label.
Particulate contamination or cloudiness may indicate deterioration.
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