Recalibration; Lm2 - HTL LABMATE Soft Manual De Instrucciones

• When pipetting liquids with temperatures different from
the ambient temperature, it is recommended to pre-
rinse the tip several times before use.
• Do not pipette liquids with temperatures above 70°C.
• When pipetting acids or corrosive solutions which emit
vapours, it is recommended to disassemble the shaft
and to rinse the piston and seal with distilled water after
finishing the pipetting operation.

8 - RECALIBRATION

HTL pipettes are calibrated by gravimetric method, using
HTL tips and distilled water, at the temperature 20±1°C,
according to EN ISO 8655 standard.
If during pipette operation you find that the accuracy
error (the difference between the real aspirated volume
and the preset volume) exceeds the permissible value
given in the table in section 1, the pipette recalibration
procedure should be carried out.
Before starting the recalibration it is necessary to check
whether the following requirements have been fulfilled
during error determination:
• the ambient temperature, and the temperature of the
pipette, tips and water was identical
• the density of the liquid used is close to that of distilled
water
• the balance with appropriate sensitivity has been used.
Volume checked [μl]
0.1 - 10
10 - 100
> 100
• mg/μl conversion factor has been taken into account
• the requirements given in sections 3 and 7 have been
fulfilled
If the above conditions are satisfied and the accuracy error
for selected volume given in section 1 exceeds the per-
missible value, the pipette recalibration procedure
should be carried out.
The recalibration can be performed within one full turn
of the key to the right or to the left only.
Recalibration conditions:
• Ambient temperature and the temperature of the
pipette, tips and liquid should be within the range 20-
25°C and stabilised during weighing within ±0.5°C
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Balance sensitivity [mg]
≤ 0.001
≤ 0.01
≤ 0.1
• Measurements should be conducted using distilled
water
• Balance sensitivity should be suitable for the volume to
be controlled
Recalibration procedure:
• Set the dose volume depending on the pipette volume
according to the following table:
Range Preset
Model of the pipette volume
volumes [μl] [μl]

LM2

0.1 - 2 0.2
LM10 0.5 - 10 0.5
LM20 2 - 20 2
LM50 5 - 50 5
LM100 10 - 100 10
LM200 20 - 200 20
LM250 50 - 250 50
LM1000 100 - 1000 100
LM5000 1000 - 5000 1000
LM10000 1000 - 10000 1000
• Perform 5 aspirations, weigh each one and calculate
the average value of the aspirations
• Calculate average aspirated volume in μl multiplying
the average aspiration amount [mg] by the distilled
water density coefficient [μl/mg], which depends on
temperature and pressure according to the following
table:
Temperature [°C]
20
21
22
23
24
25
If the average aspirated volume exceeds the permissible
value, the following should be done:
• Remove the pipetting pushbutton, (Fig. 4A),
Warning: The pipetting pushbutton consists of 2 parts:
the knob (Fig. 1A2) and the pushbutton (Fig. 1A1). After
removal of the pushbutton, both parts are separated.
ENGLISH
Volume change ΔV
Permissible for full turn of the
volumes calibration key
[μl] [μl]
(24 increments)
0.176 - 0.224 0.06
0.48 - 0.52 0.33
1.94 - 2.06 0.63
4.875 - 5.125 2.50
9.84 - 10.16 2.50
19.76 - 20.24 6.30
49.5 - 50.5 6.30
98.4 - 101.6 25.00
994 - 1006 125.00
975 - 1025 250.00
Pressure [kPa]
95.0 101.3 105.0
1.0028 1.0029 1.0029
1.0030 1.0031 1.0031
1.0032 1.0033 1.0033
1.0034 1.0035 1.0036
1.0037 1.0038 1.0038
1.0039 1.0040 1.0040
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