Use Of The Microscope In Rpc (Transmitted Light); Installing The Rpc Slider; Rpc Slider - Optika Italy IM Serie Manual De Instrucciones

11. Use of the microscope in RPC (transmitted light)

Relief phase contrast (RPC) is a modification of conventional phase contrast that leads to visible improvements
in image quality in optical microscopy. Specifically, the following parameters can be improved: contrast, focal
depth, sharpness, three-dimensionality, flatness, and halo artifacts. These effects can be achieved when the
phase rings of the condenser are replaced by slit rings.
Similar to phase contrast observation, RPC observation requires the use of a slider containing slit phase rings
and dedicated RPC objectives.
The use of the slider and objective are identical to those for phase contrast.

11.1 Installing the RPC slider

1. Insert the slider into the illumination system, printed
face up. (Fig. 51)
2. Pull the slider into the desired position, until it arrives to
the click stop.
3. When in RPC observation, keep the aperture dia-
phragm adjustment lever ① on the "O" (open) position.

11.2 RPC slider

• Two sliders are available for the use with different ob-
jectives.
• One slider is dedicated to 4X objective (Fig. 52) and
another is for 10X/20X/40X objectives. (Fig. 53)
• Both have an empty hole and a RPC ring.
SLIDER
POSITION
EMPTY empty hole ②
4x RPC ring 4x ③
10x/20x/40x
10x/20x/40x ④
MEANING APPLICATION
brightfield
observation
RPC observation
objective
RPC observation
RPC ring
with 10x, 20x and
40x objectives
①
with 4x
Page 34
② ③
② ④
F ig. 51 F ig. 51
F ig. 52 F ig. 52
F ig. 53 F ig. 53