Troubleshooting - HTL LABMATE pro LMP2 Manual De Instrucciones

Temperature [°C]
20
21
22
23
24
25
If the average aspirated volume exceeds the per missible
value, the following should be done:
• Remove the pipetting pushbutton, (Fig. 4A),
Warning: The pipetting pushbutton consists of 2 parts:
the knob (Fig. 1A2) and the pushbutton (Fig. 1A1). After
removal of the pushbutton, both parts are separated.
• Holding the volume setting knob to protect it against
rotation, insert the calibration key into the cuts of the
calibration screw, (Fig. 4B),
• Turn the key clockwise to reduce the aspirated volume,
or counterclockwise to increase the volume, (Fig. 4C).
One full turn of the calibration key changes the pipette
aspiration volume by the amount given in the table.
• Take out the key and fix the pipetting pushbutton (Fig. 4D).
The pushbutton should be fixed by placing first the knob
on the arbor (Fig. 1A2) and then the pushbutton (Fig. 1A1).
Determine the average aspirated volume. The average
volume should be within the permissible range given in
the table. If the volume exceeds the values stated, the
recalibration procedure should be repeated.
In case of pipetting the liquids with physical properties
considerably different from those of water, follow the
rules given in section 5.
More information on calibration procedure can be found
on www.htl.com.pl

9 - TROUBLESHOOTING

If you notice an improper pipette operation identify the
cause and eliminate the fault. Doing this, follow the
instruction in the sequence provided. Replacement of
elements into new ones may be required only exception-
ally, and should not occur under normal pipette use.
11
Pressure [kPa]
95.0 101.3 105.0
1.0028 1.0029 1.0029
1.0030 1.0031 1.0031
1.0032 1.0033 1.0033
1.0034 1.0035 1.0036
1.0037 1.0038 1.0038
1.0039 1.0040 1.0040
Droplets of liquid remain in the pipette tip.
• The tip is emptied too fast.
Decrease the speed of pressing the pipette push-
button.
• The tip wettability has increased due to extensive use.
Replace the tip with a new one.
Droplets of air appear in the liquid aspirated into the tip.
• The pipette tip immersion is too shallow.
Immerse the tip deeper according to the instruc-
tions.
• The pipette tip is incorrectly pressed onto the pipette
shaft.
Press the pipette firmly.
• The tip is damaged or worn out due to extensive use.
Replace the tip with a new one.
The pipette incorrectly aspirates the liquid or liquid
drops out from the tip.
• The pipette tip is incorrectly pressed onto the pipette
shaft.
Press the pipette tip firmly.
• The shaft nut is loose (Fig. 3F).
Tighten the shaft nut.
• The sealing surface of the shaft is cracked or scored.
Remove the tip ejector. Unscrew the shaft nut,
inspect the shaft and the piston assembly. Replace
the damaged parts (see sec tion 12). When reassem-
bling the pipette, the nut should be hand tightened.
In the models LMP2, LMP10 and LMP20, the damage of
the shaft may also cause a damage of the piston assem-
bly. Replace the damaged parts (see section 12).
When reassembling the pipette, the nut should be
hand tightened.
To remove the tip ejector in models LMP5000 and
LMP10000, remove the ejector pushbutton (Fig. 3N)
and using a screwdriver unscrew the tip ejector by
turning the screwdriver counterclockwise.
• Damage of the piston or seal due to prolon ged use with
the aggressive liquids.
Disassemble the pipette as described above.
Replace the piston, seal and O-ring (see section 12).
Rinse the inside of the shaft in distilled water and dry.
Lubricate the seal and O-ring with the lub ri cant.
The replacement of the piston requires conduc ting
of calibration procedure.
ENGLISH
12