Cleaning And Sterilization - HTL LABMATE pro LMP2 Manual De Instrucciones

Note: The parts of LMP2 and LMP10, pipette should
be lubricated evenly with the minimum amount of
lubricant.
• The pipette is reassembled improperly.
Disassemble the pipette and reassemble it, obser -
ving the proper sequence of steps (Fig. 3).
• No lubricant on the sealing elements.
Remove the tip ejector. Unscrew the shaft nut,
remove the shaft, piston assembly, seal and O-ring.
Rinse the removed parts in distilled water and dry
thoroughly. Lightly lubricate the inside surfaces of
the seal and the O-ring with the lubricant.
Reassemble the pipette in the reverse order.
• Contamination of the inside of the pipette caused by
extensive aspiration of chemically aggre ssive liquids or
because liquid got inside the pipette.
Remove the tip ejector. Unscrew the nut, remove
the shaft, piston assembly, seal and O-ring. Rinse
the removed parts with distilled water and dry thor-
oughly. Lightly lubricate the inside surfaces of the
seal and the O-ring with the lubricant. Reassemble
the pipette in the reverse order.
Note: All parts may be autoclaved at a temperature of
121°C for 20 minutes at pressure 1 bar.
The shaft of the 5000 and 10000 models should be
autoclaved without the filter.
The handle and the outside of the pipette may be
cleaned using a cloth damped in isopropanol.
If the pipette malfunction persists after carring out the
above steps, send the pipette to your HTL service repre-
sentative.
Before returning the pipette, make sure that it is free from
any chemical, radioactive or microbiological contamina-
tion that might pose any danger during transportation and
repairing. Clean the pipette as thoroughly as possible.
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10 - CLEANING AND STERILIZATION

Cleaning:
External surfaces of the pipetting pushbutton, the ejector
pushbutton, the handgrip, the shaft nut and the adjust-
ment knob may be cleaned using a cloth dampened in
isopropyl alcohol. The remaining parts removed from the
pipette during pipette disassembly may be washed with
distilled water or isopropyl alcohol.
Warning:
Before using cleaning agents other than those recom-
mended by the manufacturer, check the compatibility
charts and consider chemical resistance of the following
plastics: PP, PC, POM, PA, PPS, PVDF used to produce
the pipette parts.
Sterilization:
The pipette can be sterilized in the autoclave at 121°C for
20 minutes. After sterilization, the pipette should be dried
and cooled to room temperature.
It is recommended:
- to sterilize the pipettes in autoclave with an initial vacu-
um and drying cycle,
- prior to sterilization unscrew the shaft nut slightly in the
LMP2-LMP1000 pipettes, and unscrew the shaft slight-
ly in the LMP5000 and LMP10000. After autoclaving
these parts should be screwed tight again.
The precision of the results should not alter if the pipet-
ting process and autoclaving are carried out as
described in this manual. Because a slight change in the
accuracy of the dosage may occur, it is recommended to:
- check the calibration of the pipette after the initial first,
third and fifth autoclaving cycles and then after every 10
autoclaving cycles.
The pipettes are UV resistant which was confirmed by
our tests. The recommended distance from the radiation
source to exposed element should be not less than
50 cm. Prolonged and very intense UV exposure
can cause de-coloration of pipette parts, without affect-
ing its performance.
ENGLISH
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