Use Of The Microscope (B-383Fl); Use Of The Microscope (B-383Ld) - Optika Italy B-380 Serie Manual De Instrucciones

14.5 Use of the microscope (B-383FL)

1. Turn on the power supply for the mercury bulb and wait 5
minutes for the arc to stabilize.
2. Move the filter selector ① to one of the 2 available positions
until the click stop. (Fig. 49).
3. The microscope has a 3-position filter holder. The leftmost
position allocates the B filter, the central position is empty
for transmitted light observation and the rightmost position
allocates the G filter.

14.6 Use of the microscope (B-383LD)

1. Turn on the fluorescence LED, by switching on "II" the main
switch placed on the back side of the frame. (Fig. 50)
2. Move the filter slider
FILTER EXCITATION
CUBE FILTER
B 475/30 nm
G 530/40 nm
② all the way in. (Fig. 51).
DICHROIC
MIRROR
505 nm
570 nm
EMISSION
FILTER
• FITC: fluorescent antibodies
515LP nm • Achridine orange: DNA, RNA
• Auramine
• Rhodamine, TRITC: fluorescent antibodies
590LP nm • Propidium iodide: DNA, RNA
• RFP
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①
②
APPLICATIONS
F ig. 49 F ig. 49
F ig. 50 F ig. 50
F ig. 51 F ig. 51