Puritan Diagnostics UniTranz-RT Manual Del Usuario página 5

Table 1 – Recovery of Viruses (continued)
Dilution
Organism of Neat
Parainfluenza 3
Respiratory
Syncytial Virus
Varicella-Zoster
Virus
From each dilution, 100 µL were inoculated onto test swab tip followed by placement of the swab into the test
a
device containing 3 mL of transport medium
Average of triplicate tests (± standard deviation) performed on 200 µL of test device medium at each time point;
b
RT, room temperature
Table 2 – Recovery of Chlamydia
Dilution
Organism of Neat
Stock
Chlamydia
pneumoniae
1:100
Chlamydia
trachomatis
1:100
From each dilution, 100 µL were inoculated onto test swab tip followed by placement of the swab into the test
a
device containing 3 mL of transport medium
Average of triplicate tests (± standard deviation) performed on 200 µL of test device medium at each time point; RT,
b
room temperature
Percent
Infectivity of
Host Cells
Stock a
(%) Infectivity)
1:10 3%
1:100 25%
1:10 76%
1:100 100%
1:10 100%
1:100 100%
Percent
Infectivity of
Host Cells
a
(% Infectivity)
1:10 100%
100%
1:10 100%
100%
Incubation
Storage
Time Prior
Time
to Reading
(Hours) (Hours)
0
24 48
48
0
24 48
48
0
24 24
48
0
24 24
48
0
24 24
48
0
24 24
48
Incubation
Storage
Time Prior
Time
to Reading
(Hours) (Hours)
0
24 48
48
0
24 48
48
0
24 48
48
0
24 48
48
Mean Viability of Test Organism
Using Test (Puritan) Device: Foci
Counts with SD
b
4°C
501 ± 116 501 ± 116
30 ± 10 628 ± 208
101 ± 26 107 ± 56
358 ± 87 358 ± 87
24 ± 10 292 ± 60
47 ± 13 54 ± 23
140 ± 19 140 ± 19
176 ± 20 170 ± 14
78 ± 24 131 ± 26
25 ± 6 25 ± 6
74 ± 15 62 ± 5
59 ± 19 74 ± 4
325 ± 91 325 ± 91
253 ± 51 212 ± 43
33 ± 13 117 ± 47
132 ± 45 132 ± 45
97 ± 12 97 ± 3
87 ± 69 94 ± 49
Mean Viability of Test
Organism Using Test (Puritan)
Device: Foci Counts with SD
b
4°C
169 ± 33 169 ± 33
356 ± 70 456 ± 68
301 ± 121 345 ± 66
65 ± 6 65 ± 6
163 ± 25 134 ± 35
110 ± 24 131 ± 33
227 ± 63 227 ± 63
204 ± 79 627 ± 197
184 ± 62 234 ± 102
73 ± 10 73 ± 10
60 ± 12 138 ± 50
57 ± 19 92 ± 32
RT
RT
5