Tabla de contenido
CAUTION: Do not jar or bump the
gel box once the lid is place.
The electrical connection is
made by gravity once the lid is in
position. While this design helps
to minimize sample disturbance
during lid placement, it also may
result in a disruption of power
to the unit if the lid or unit are
disturbed during the run.
CAUTION: DO NOT EXCEED THE
MAXIMUM OPERATING VOLTAGE
OF 150 VOLTS.
G. To Pause a Run and/or Change Parameters
1
To pause the run select Pause. During the pause mode
the voltage amperage or time can be changed by
highlighting the function and using the arrow keys then
pressing enter. Once the changes have been made the
start button can be pressed to resume the run.
2
To stop the run press the pause button for 30 seconds.
Stop will appear.
H. Sample Electrophoresis
1
The maximum suggested applied voltage for the
electrophoresis of DNA in agarose gels using the
Hoefer HE-PLUS Gel System is 150 volts.
2
In a 1% TBE gel, this translates into a run time of
approximately 1 hour. Lower voltages may be used,
of course, and as a general rule, a 70 V run will take
twice as long as a 145 V run. Higher voltages may
be used to decrease run time, however, if the unit is
being operated at higher voltages than 150 V, the heat
generated during electrophoresis may decrease sample
resolution. Such artifacts may be avoided by running
the unit in a cold room or adding 1X electrophoresis
buffer "ice cubes" to keep the unit properly cooled.
3
Follow the sample migration into the gel using the
loading dye as an indicator. (See page 12 for the
Sample Loading Buffer recipe.) Allow the samples to
migrate until the fragments have separated, normally
until the bromophenol blue dye front has migrated ¾
of the way down the gel.
•
p9
Tabla de contenido
