Tabla de contenido
•
p2
1
Make 500 ml of either 1X TAE or 1X TBE
electrophoresis buffer.
2
Weigh an appropriate quantity of agarose (see
Table 1) and place it into a 250 ml flask. Add a
sufficient quantity of either 1X TAE or 1X TBE buffer
(prepared in step 1) to achieve a final volume of
100 ml agarose solution.
Table 1: Gel Concentrations and Resolving Ranges
Concentration
of Agarose in
Gel (%w/V)
0.3
0.6
0.7
0.9
1.2
1.5
2.0
Table taken from Sambrook, J., Fritsch, E.F., & Maniatis, T. (1989)
Molecular Cloning, A Laboratory Manual, 1, 6.8 613.
Agarose (g)
Efficient Range
per 100 ml
of Separation
Buffer
of Linear DNA (Kb)
0.3
5 – 60
0.6
1 – 20
0.7
0.8 – 10
0.9
0.5 – 7
1.2
0.4 – 6
1.5
0.2 – 3
2.0
0.1 – 2
Tabla de contenido
