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B. Casting the Gel
1
Place the gel casting stand on a lab bench.
2
Insert the gel casting tray into the casting stand. If
you are using the 6 × 12.5 cm gels place the spacer
in the center of the casting stand, then insert the two
6 × 12.5 cm landscape gel trays.
3
When the gel solution has cooled to approximately
55 °C, slowly pour it into the gel tray. If hotter gel
solutions are routinely poured, the tray may warp
over time.
4
If bubbles form on the surface of the gel upon
pouring, use the comb to either pop them or lightly
brush them to the sides of the gel. If large bubbles
are allowed to harden within the gel, they may cause
artifacts to occur during electrophoresis.
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