Manuales relacionados para Varian 212-LC
Resumen de contenidos para Varian 212-LC
- Página 1 Varian, Inc. 2700 Mitchell Drive Walnut Creek, CA 94598-1675 U.S.A. Varian 212-LC Chromatography Pump Operation Manual ©Varian, Inc. 2006 Printed in U.S.A. 03-954103-00:Rev. 1...
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Página 2: Tabla De Contenido
Contents Description ........................1 Specifications ........................ 2 System ................................2 Pump ................................2 Standalone Capabilities ..........................2 Unpacking and Installation................... 3 Installing the Piston Wash Seals........................8 Standalone Operation ....................10 Priming the Pump............................10 Setting Under-pressure Limit (P Min)......................11 Setting Flow Rate............................ -
Página 3: Description
User Interface for Stand-alone Operation. The 212-LC is intended for use with Varian MS Systems under MS Workstation control. This pumping system is optimized for use in gradient or isocratic mode at flow rates below 2 mL/min. -
Página 4: Specifications
Specifications System Dimensions: 10.25 in. (26 cm) wide x 13 in. (33 cm) high x 14 in. (36 cm) deep Weight: 32 lbs (14.5 kg) Power Input: 85 – 264 Vac; 47 – 63 Hz; 60 Watts Pump Flow Rate: Programmable from 0.001 µL/min to 2 mL/min Maximum Pressure Setting: 6,000 psi (Default = 5,400 psi) Maximum Pressure Capacity: 6,000 psi at 2 mL/minute as indicated on the pump display panel... -
Página 5: Unpacking And Installation
Unpacking and Installation The 212-LC System consists of two high pressure HPLC pumps. Pump A serves as the master control pump and will also be referred to as the pump which delivers solvent A. To distinguish the A and B pumps, the Serial Number for the A pump is labeled as AXXXX, whereas the B pump is labeled as BXXXX. - Página 6 Check Valve 1/16” Transfer Tube Figure 1 Varian 212-LC Chromatography Pump HPLC System, Front View 5. Using the 0.005” ID PEEK® tubing provided, cut two lengths of tubing to connect each pump outlet port to one each of the mixer inlet ports.
- Página 7 Number Label Label Figure 2 212-LC Chromatography Pump System, Rear View 10. Turn the power on (both pumps) with the main switch at the rear left side of chassis. The red LED lamp located on the right side of the key pad should come on, indicating that the pump is now ready for operation.
- Página 8 6-32 x 3/8” Mixer Inlet Port Mixer 03-911805-01 Knob R0-028000-86 Mixer Outlet Port Mixer Bracket Mixer Inlet Port Fitting, Fingertight (outlet ports) 0.005” ID Waste Line PEEK Tubing Screw, 8-32 x 3/8” Figure 3 212-LC with Mast Installed, Front View...
- Página 9 Screw 8-32 x 3/8” Power Cord Cable, Null Modem 81-839602-00 Cable, RS232 Figure 4 212-LC with Mast Installed, Rear View...
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Página 10: Installing The Piston Wash Seals
Installing the Piston Wash Seals The 2 mL pump heads supplied with the Varian 212-LC pumps are capable of being configured for washing the back of the piston. This is important when pumping with a mobile phase containing a high concentration of buffer. - Página 11 Flush Solvent Pump A 36 in. Teflon Tubing Lower Flush Port 12 in. Teflon Tubing Lower Flush Port Pump B Waste Solvent Lower Flush Port Side Flush Port 10 ft Teflon Tubing Figure 5 Wash Seal Option Connections...
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Página 12: Standalone Operation
Stand-alone Operation This section describes the use of the pump in Stand-alone mode, via the local keypads. Some of the information is also relevant to the operation under MS Workstation Control, in particular Priming, customizing the solvent configuration, and maintenance tasks. Priming the Pump Refer to the keypad layout in Figure 6 for key orientation and description. -
Página 13: Setting Under-Pressure Limit (P Min)
Setting Under-pressure Limit (P Min) Refer to the keypad layout in Figure 6 for key orientation and description. 1. The default setting for P Min is 0 psi. Once P Min is set, this setting becomes the default value. If P Min has a positive value and the pump is started, the prompt “Reset MIN Value? Yes”... -
Página 14: Setting Flow Rate
5,400 psi 6,000 psi Solvent Compressibility Compensation The 212-LC contains a library of solvents and their solvent compressibility factors can be selected to automatically compensate for changes in solvent compressibility. The solvents appear on the screen in their abbreviated names as shown in the table on page 13. Relying on the factory default value for solvent compressibility will result in ±... -
Página 15: Selecting A Solvent
(CF) for solvents other than those listed can be found below. Remote Control The 212-LC Pump System can be controlled remotely in one of two ways. External contact closures enable the system to be controlled by most HPLC autosamplers. For instructions see page 18. The 212-LC Pump System can also be controlled from a remote computer via the RS232 interface using MS Workstation version 6.6 SP1 and above. -
Página 16: Determining Compressibility Factors
Determining Compressibility Factors Use the following procedure to calculate the compressibility factor for solvents not listed in the solvent library. In general, the more compressible the solvent, the higher the compressibility factor needs to be, for example water is 100, heptane is 200. 1. -
Página 17: To Delete A Method
5. Enter the number of the method that will be over-written and press Enter. Alternatively, the keys can also used to input the number. 6. The display will request confirmation by pressing Enter again. Once the copy is completed, the display will return to the Main Operating Menu. -
Página 18: Edit Step Function
Edit Step Function The second field on the method screen is Step00. Enter the step size (% step) or use the keys to change the step size and press Enter. Enter 0 if you do not want a step gradient. The step size you enter will determine the percentage change in solvent B and the gradient time will determine when each step will occur. -
Página 19: Running And Stopping A Method
Running and Stopping a Method Press Activate. Enter the number of the method file you wish to run (00 – 25), press Enter and then press Run. This process will start the method file at T0 conditions (times out after 60 minutes) until Run is pressed again or the “Start”/”Common”... -
Página 20: Remote Control By External Contact Closure And Autosampler Control
(T0) will display the time in 0.10 minute increments. The 212-LC system is now in the “Idle” condition and is ready to run the method file. The pump returns to “Idle” after running each method file. -
Página 21: Operation With Ms Workstation
Methods are executed in the driver, and simple flow commands are sent to both pumps. The 212-LC MSWS Method can contain up to 100 steps specifying flow and composition, with a time resolution of 1 second. The method always executes a linear ramp of flow and composition from one step to the next. -
Página 22: Connecting The Pumps
• Follow the on-screen instructions until the installation is complete. To verify that the 212-LC driver is present, right-click on the right side of the Workstation toolbar and select Enable/Disable Instrument Modules. In the resulting dialog, verify that the 212-LC driver is listed in the enabled or disabled modules. -
Página 23: Configuring The Software
Drag the module into the Instrument, then switch to the instrument Window (press Alt+1 or use the Windows menu). You are ready to use the 212-LC under WS Control. Refer to the on-line help in System Control and in the Method Editor for... -
Página 24: General Maintenance Information
These repairs are typical of many pumps used in Liquid Chromatography and are described in detail in this section. Please contact your local Varian office to arrange service calls by our certified field service representatives for additional services regarding the pumps. -
Página 25: Inlet Check Valve Cartridge
Inlet Check Valve Cartridge Remove the finger-tight fitting on the inlet line and follow the same general directions for replacing the outlet cartridge. Be sure the arrow on the cartridge is aligned with the flow direction. Spring Housing/ Pump Head Assembly Inlet Housing/Cartridge Outlet Check Valve Outlet Housing... -
Página 26: Replacing The Piston Seal Assembly
Replacing the Piston Seal Assembly Replacement of the Piston Seal Assembly is recommended every six months. Using the open end wrench (provided) remove the 1/16” transfer tube which connects the output of the pump head to the purge valve body (Figure 1). Using the 9/64”... -
Página 27: Replacing The Wash Seal Assembly
Push the new piston seal assembly over the end of the exposed ceramic piston with the fingertip or thumb, but do not allow your fingernails to contact the seal as damage may occur. This procedure aligns the piston seal assembly on the ceramic piston. It is normal for the seal not to push completely over the end of the ceramic piston. -
Página 28: Replacing The Ceramic Pistons
Spring Housing Wash Seal Assembly Teflon Piston Wiper Alignment Pins Pump Head Manifold Wash Seal Assembly Pump Head Piston Seal Assembly Figure 12 Wash Seal Assembly Replacement Replacing the Ceramic Pistons Every six months, inspect the ceramic pistons. The complete spring housing/pump head assembly (pump head and manifold) must be removed from the pump when the ceramic pistons are being inspected and/or replaced. - Página 29 Frame Drive Casting Spring Housing Alignment Pins Ceramic Piston Assembly Figure 13 Spring Housing Replacement To reinstall the ceramic pistons, push the ceramic pistons back into the spring housing through the rear bushings using care to avoid scratching the ceramic surface. Tighten the spring housing to the frame drive casting and install the wash seal assemblies, manifold, piston seal assemblies, and pump head following the procedures previously described.
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Página 30: Troubleshooting
Dirty inlet check valve MeOH for 1 minute, maximum. Blow air through in direction of arrow and reinstall. Dirty inlet check valve Replace with new check valve. Varian uses the same check valve cartridge for both inlet and outlet. Clogged solvent filter Replace with new filter. - Página 31 Problem Cause Remedy Seal life unusually Worn or scratched piston Replace with new piston. short Build-up of salts on piston Always run DI water through the pump before shutting down for the day when running buffers. Mobile phase incompatible Use a Teflon® seal. with seal material Frequent check Contaminated solvent...
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Página 32: Replacement Parts
Replacement Parts Varian Part No. Item Description 391180101 Model 212-LC, Pump A 391180201 Model 212-LC, Pump B 391180001 Model 212-LC, Gradient System AS500112702 2 mL Piston Seal Wiper AS5002117 Injection Valve Mounting Bracket AS500213501 Solvent Inlet Line Assembly for Micro Pumps... -
Página 33: Wiring Diagram
Wiring Diagram... - Página 35 Customer to return the defective workmanship for the periods specified and in accessory or instrument to Varian or to send it to a accordance with the terms on the face of Varian's designated service facility.
- Página 36 Garan- garantie wird die Reparatur der Instrumentstörungen tien der Verkäuflichkeit und Eignung für einen be- sein, die sich nach Varian's Ansicht auf Defekte in den sonderen Zweck, Gebrauch oder Anwendung und Originalteilen oder bei der Herstellung zurückführen läßt...
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Página 37: Limitation De Garantie
Varian diffusera des mises à jour des de Varian au client d'origine. Cependant, lorsque le logiciels, le cas échéant, et si de l'avis de Varian, elles client a acquitté les frais d'installation ou que celle-ci est constituent la mesure corrective la plus appropriée en la inclue dans le prix d'achat, la période de garantie... - Página 38 In assenza d'un contratto di licenza e salvo diverso magnetico, fotomoltiplicatori, filamenti, guarnizioni per accordo scritto tra la Varian e il Cliente, vale il periodo di vuoto, e tutte le parti esposte all'azione dei campioni o garanzia indicato nell'offerta della Varian. La Varian delle fasi mobili.
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Página 39: Productos Hardware
Esta garantía no tendrá efecto en los casos de accidente, Varian, u otro tipo de hardware en el que Varian certifique abuso, alteración, utilización incorrecta, negligencia, que funcionan según lo descrito en Manual de rotura, mantenimiento o uso inadecuados, modificaciones instrucciones, y que esté... - Página 41 NOTES CAUTIONS WARNINGS accordance with this instruction manual and any additional information which may be provided by Varian. Address any questions regarding the safe and proper use of your equipment to your local Varian office. NOTE Information to aid you in obtaining...
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Página 42: General Safety Precautions
It is the responsibility of the Customer to inform Varian Customer Support Representatives if the instrument has been used for the analysis of hazardous biological, radioactive, or toxic samples, prior to any instrument service being performed or when an instrument is being returned to the Service Center for repair. - Página 43 Flash Chromatography GC Safety Practices The operator should be familiar with the physico- Exhaust System chemical properties of the components of the mobile No special exhaust ducting is necessary for GC phase. detectors installed in a well-ventilated room except Keep solvents from direct...
- Página 44 Varian, Inc. Analytical Instruments Sales Offices For Sales or Service assistance and to order Parts and Supplies, contact your local Varian office. Argentina France...
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Página 45: Sicherheitsinformationen
HINWEIS ACHTUNG WARNUNG, Sie Ihr Gerät in Übereinstimmung mit dieser Arbeitsanleitung und allen möglichen zusätzlichen Informationen von Varian betreiben. Alle Fragen bezüglich Sicherheit und Handhabung Ihres Gerätes richten Sie an Ihr Varian Büro. ACHTUNG WARNUNG HINWEIS Eine Information, um einen optimalen Weist auf Situationen, die zu mäßiger... -
Página 46: Allgemeine Sicherheitsmaßnahmen
Es muß mit geeigneten Gasen und/oder Lösungsmitteln und innerhalb der im Handbuch spezifizierten maximalen Werte für Druck, Flüsse und Temperaturen betrieben werden. Der Kunde ist vor der Durchführung irgendeines Geräteservices verpflichtet den Varian WARNUNG Kundendienstvertreter zu informieren, wenn das Instrument für Analysen gefährlicher biologischer, radioaktiver oder toxischer Proben benutzt worden ist. - Página 47 Öffnen Sie niemals eine unter Druck stehende GC Sicherheitspraktiken Lösungsmittelleitung oder ein Ventil. Halten Sie Abgassystem zuerst die Pumpe an und lassen Sie den Druck auf Für GC Detektoren, die in einem gut durchlüfteten Null abfallen. Raum installiert sind, ist keine spezielle Abgasführung Benutzen Sie splittersichere Reservoirs, die für erforderlich, außer wenn die Detektoren zum Testen einen Druck von 3,4 bis 4,1 bar ausgelegt sind.
- Página 48 Verfügbarkeit von Ersatzteilen Serviceverfügbarkeit Varian bietet seinen Kunden auch Es ist Varian’s Grundsatz, Ersatzteile für alle Instrumente und die wichtig- nach dem Auslaufen der Garantie sten Zubehöre für einen Zeitraum von fünf (5) Jahren nach dem Fertigung- eine Vielfalt von Serviceleistungen sauslauf dieser Geräteserie verfügbar zu haben.
- Página 49 AVERTISSEMENTS. matériel conformément aux instructions du présent manuel et à toute autre information émanant de Varian. S’adresser au bureau régional Varian pour toute question relative à la sécurité ou à l’utilisation correcte du matériel. NOTE Information destinée à tirer le Attire l’attention sur une situation...
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Página 50: Précautions Générales En Matière De Sécurité
Le client est tenu d’informer le service Varian d’assistance à la clientèle que son matériel ATTENTION a été utilisé pour l’analyse d’échantillons biologiques dangereux, radioactifs ou toxiques avant que n’en soit effectué... -
Página 51: Mesures De Sécurité En Cpg
Ne jamais déconnecter un conduit de solvant ou Mesures de sécurité en CPG une vanne sous pression. Arrêter préalablement la Système d’échappement pompe et laisser la pression descendre à zéro. Les détecteurs CPG installés dans une pièce bien Utiliser des réservoirs incassables à 50-60 psi. ventilée ne nécessitent pas de conduits spéciaux d’échappement excepté... - Página 52 Points de vente des instruments analytiques Varian Contactez votre point de vente régional Varian pour toute question commerciale ou de service d’assistance à la clientèle ou pour passer commande de pièces et de fournitures.
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Página 53: Informazioni Sulla Sicurezza
. E’ importante che lo strumento venga utilizzato rispettando le istruzioni fornite in questo CAUTELA ATTENZIONE manuale o che verranno fornite successivamente dalla Varian. Per ogni eventuale chiarimento sull’uso o sulla sicurezza, si prega di contattare la Varian di Leinì (TO). ATTENZIONE... -
Página 54: Norme Di Sicurezza
E’ responsabilità del Cliente informare il Servizio Tecnico Varian, prima di qualsiasi ATTENZIONE intervento di riparazione, se lo strumento è stato utilizzato per l’analisi di campioni biologicamente pericolosi, radioattivi o tossici. - Página 55 Procedure di Sicurezza in GC Non smontare mai una linea del solvente od una Scarico dei Gas valvola quando il sistema è sotto pressione. Per i rivelatori GC non è richiesto alcun sistema Fermare prima la pompa ed aspettare che la particolare di scarico dei gas, se lo strumento è...
- Página 56 Disponibilità delle Parti di Ricambio Servizi Tecnico E’ politica della Varian il fornire le parti di ricambio per lo strumento ed La Varian, alla scadenza del periodo i suoi accessori per un periodo di cinque (5) anni a partire dalla data di di garanzia, è...
- Página 57 PRECAUCION ATENCION instrumento de acuerdo con este Manual de Operación y cualquier otra información que le proporcione Varian. Remita a la Oficina Local de Varian cualquier cuestión que tenga respecto al correcto uso de su equipo. ¡PRECAUCION! ATENCI N Ó...
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Página 58: Precauciones Generales De Seguridad
Debe ser operado usando gases y/ó disolventes apropiados y con unos niveles máximos de presión, flujos y temperaturas, según se describe en este manual. El Usuario tiene la obligación de informar al Servicio Técnico de Varian cuando el ATENCI N Ó... -
Página 59: Gc Prácticas De Seguridad
Nunca abra una línea ó una válvula bajo presión. GC Prácticas de Seguridad Apague la bomba antes y deje que la presión baje a Sistema de Extracción cero. No se necesita un sistema de extracción para los Utilice depósitos irrompibles que sean capaces de detectores GC instalados en un laboratorio bien operar a 50-60 psi. -
Página 60: Disponibilidad De Recambios
Disponibilidad de Recambios Disponibilidad de Servicio Es Política de Varian disponer de Recambios para cualquier instrumento y la mayoría de los accesorios por un periodo de cinco (5) años después del Varian ofrece una gran variedad de último instrumento fabricado. Los recambios durante esos cinco años sistemas de Servicio para mantener estarán disponibles, pero siempre bajo el sistema “Según disponibilidad”.