Pipetting Recommendations; Aspirating And Dispensing Liquids; Aspirating Liquids; Dispensing Liquids - HTL DISCOVERY PRO DP2 Manual De Instrucciones

3. PIPETTING RECOMMENDATIONS

Observing the following recommendations will ensure
accuracy and repeatability of liquid sampling.
• While operating DISCOVERY PRO pipette the volume
setting should be locked - the adjustment knob in its
lower position (fig. 2C).
• Pipette is equipped with a switch of counter's mode of
operation – calibration switch (fig. 1E). This switch
should be shifted only for the time of calibrating the
pipette (upper position marked with the "CAL" symbol).
• Operate the pipette pushbutton slowly and smoothly
when liquid aspiring and dispensing.
• The depth of tip immersion in the aspired liquid should
be kept at necessary minimum, and should remain
constant during aspiration (Table 2).
• While operating, the pipette should be held in a vertical
position.
• Each new tip should be pre-rinsed.
• The tip should be replaced with a new one if:
- a different type of liquid is handled;
- a different liquid volume is dispensed;
- visible droplets of liquid remain in it.
• Pipetted liquid should not enter the pipette shaft. To
ensure this:
- press and release the pipetting pushbutton slowly
and smoothly;
- do not put the pipette away if there is any liquid in the
tip;
- do not invert the pipette;
- do not set and aspire volumes exceeding nominal
values.
• Before aspiring liquid of temperature that differs from
ambient temperature, it is recommended to rinse the
tip several times with the aspired liquid.
CAUTION: After pipetting acids and aggressive solu-
tions, it is recommended to unscrew the pipette and
rinse the pipette plunger, seal and shaft with distilled
water.
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4. ASPIRATING AND DISPENSING LIQUIDS

4.1 ASPIRATING LIQUIDS

A B C
Fig. 4
• The pipetting pushbutton (fig. 1A) should be pressed to
the first stop (fig. 4A),
• While holding the pipette in a vertical position, immerse
the tip into the aspired liquid. Depth of the tip immer-
sion depends on the pipette model. The recommended
values are shown in Table 2. The tip may aspire air if
immersed to a depth lower than recommended or if the
pipetting pushbutton is released quickly,
• To aspire liquid release the pipetting pushbutton
smoothly (fig. 4B),
• Wait for about 1 second before withdrawing the tip
from the aspired sample (fig. 4).
WARNING! Do not touch the used tip.
Volume
Depth
Table 2

4.2 DISPENSING LIQUIDS

• While holding the pipette slightly inclined from 10 to 40°
off the vertical, place the tip orifice against the inside
wall of the receiving vessel.
• Then, press the pipetting pushbutton smoothly to the
first stop, dispensing the liquid (fig. 4C).
ENGLISH
D E F
2,10 μl 20, 100 μl 200, 1000 μl
<1mm 2-3mm 2-4mm
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