Levenhuk MED PRO 600 Fluo Guia Del Usuario página 15

• observing the object image, focus the condenser, moving it along the height with the knob (fig. 3, 5) for sharp image of
the iris field diaphragm;
• if the field diaphragm image is displaced, bring the image into the field center with condenser alignment screws (fig. 3, 2);
• open the field diaphragm with a ring (fig. 1, 23) along the eyepiece field diameter — so that edges of the iris diaphragm
are slightly beyond the eyepiece field;
• remove the eyepiece from the right eyepiece tube;
• observing the exit pupil image in the right tube, open the aperture diaphragm of the condenser with the knob (fig. 3, 1)
to the exit pupil size. Make sure that the lamp filament image fills the eye. If the image is displaced by the lamp position
adjustment knobs (fig. 1, 14 and 15), align the filament image. Using the collector adjustment knob (fig. 1, 16), fill the
objective exit pupil with light;
• install the eyepiece in the right eyepiece tube.
Normal operation of the illumination system is provided only when slides with thickness of 1—1.2mm are used.
Microscope focusing for binocular observation
Focus the microscope on the object when observing through a binocular tube as follows:
• put the object on the stage (fig. 2, 10) of the microscope;
• put the objective lens with the necessary magnification into the path of rays;
• rotating the coarse focusing knob (fig. 2, 13), carefully lift the stage to the distance of 0.5mm to the objective lens;
• observing with your right eye into the eyepiece installed into the right eyepiece tube, slowly put the stage down,
rotating the coarse focusing knob (fig. 2, 13). As the object contours appear, focus the microscope using a fine focusing
knob (fig. 1, 19 or fig. 2, 12);
• observing with your left eye (the right eye is closed) into the eyepiece installed in the left eyepiece tube, get the sharp
image of the object by rotation of the diopter mechanism ring (fig. 2, 4). Do not touch the focusing mechanism knobs
when doing this;
• set the distance between axes of eyepiece tubes of the binocular head in accordance with the observer's eye base
by rotation of the casings with eyepiece tubes in respect to the hinged joint axis so that images of the object in each
eyepiece of the head when observing with two eyes are perceived by the observer as one;
• start investigating the slide.
To achieve the best image quality, it is recommended to close the aperture diaphragm of the condenser by 1/3 of the
objective exit pupil for each objective lens.
Selection of objective lenses
It is recommended to investigate the object from the objective lens of the lowest magnification, which is used as a search
objective lens when selecting a site for more detailed investigation.
After the site for investigation was selected, put its image into the microscope field center. If this operation is performed
not carefully enough, the object site of interest for the observer may not get into the field of the stronger objective lens as
magnifications are changed.
Then you can go to work with stronger objective lenses, including an objective lens for oil immersion.
Immersion objective lens handling
Handle the immersion objective lens in the premises with temperature from +15 to +25°С (59 to 77°F). Use immersion oil
with refraction index nD = 1.515.
WARNING! DO NOT USE SUBSTITUTES INSTEAD OF IMMERSION OIL AS IT MAY SIGNIFICANTLY WORSEN IMAGE
QUALITY.
Prior to immersion objective lens handling, set up the microscope as specified in subsections "Halogen lamp activation and
illumination setup" and "Selection of objective lenses" and clearly identify the object site for more detailed investigation.
To handle the immersion objective lens:
• lower the stage with a knob (fig. 2, 13);
• apply immersion oil on the object;
• carefully lift the stage using coarse focusing knobs (fig. 2, 13) until the objective lens contacts with the immersion drop
on the object;
• observing into the eyepieces and using the fine focusing knob (fig. 1, 19 or fig. 2, 12), get the sharp image of the
examined object.
If images of air bubbles that may be contained in the immersion oil layer appear in the eyepiece field in process of focusing,
use coarse focusing knobs (fig. 2, 13), put the stage down and repeat focusing.
To investigate the objects, it is necessary to make sure that the iris diaphragm of the objective lens 100х/1.3 is open.
To increase the image contrast, first adjust the condenser aperture diaphragm with a knob (fig. 3, 1), then perform finer
adjustment of the contrast with the objective lens iris diaphragm.
Upon completion of operation, remove immersion oil from the frontal objective lens with blotting paper and wipe the
contaminated surfaces with cotton wrapped on a stick and slightly soaked in ether or alcohol mix.
Do not push on the frontal objective lens when cleaning.
If the image contrast has lessened or sharpness disappeared as a result of wrong handling of the immersion objective lens,
the following is recommended:
• unscrew the objective lens, clean the frontal lens as shown above;
• using oblique light from a table-lamp and a magnifying glass, make sure that there is no dirt, traces of immersion oil, cracks
or dents on the frontal lens surface;
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