User Calibration Procedure; Fine Adjustment; Troubleshooting; General Maintenance - VWR Zippette Classic Manual De Instrucciones

USER CALIBRATION PROCEDURE

Fine Adjustment

The figures quoted on our Test Certificate apply to the calibration of the
Zippette Classic at full volume. The Zippette Classic adjustment cap enables
the user to fine calibrate for accuracy. Using the adjustment disc tool that is
held on the rear of the Zippette Classic body, change the factory calibration
by turning the slot on the printed top cap insert:-
Anti-clockwise to increase volume Clockwise to decrease volume
The graduations on the adjustment ring increase or decrease by the
following volumes:-
Description
Zippette Classic 50 ml 0.1 ml per line
30 ml 0.1 ml per line
10 ml 33 µl per line
5 ml 17 µl per line
2.5 ml 8 µl per line

Troubleshooting

Problem Possible cause
Air bubbles appear in Liquid reservoir is empty
dispense tube
Too fast filling action
Glass barrel is not sealing
against FEP 'O' ring
Leaking piston
Leaking discharge valve
Barrel does not fill Inlet tube not fitted correctly
with liquid
Inlet valve stuck
Glass barrel is not sealing
against the FEP 'O' ring
Dispensing not possible Blocked dispense tube nozzle Disassemble the dispense tube and
Discharge valve stuck
Wrong dispense volume Instrument not calibrated
Leaking valves
Liquid appears between Glass barrel is not sealing
glass barrel and against the FEP 'O' ring
polypropylene protection
sleeve
Damaged FEP 'O' ring

General Maintenance

Maintenance/Cleaning

Note; All maintenance should be carried out wearing suitable eye protection
and protective clothing. If in doubt, consult your safety officer.
1. Make sure that the Zippette Classic is completely empty and turn the
anti-drip tap to 'Open' position.
Remedy
Refill reservoir and prime unit
Pump liquid out, refill and dispense
slower
Unscrew the threaded platform
ring and make sure that the FEP 'O'
ring is properly fitted into its recess
if necessary stretch 'O' ring slightly
using fingertips
Clean piston. If problem persists,
replace piston assembly (21)
Clean unit by flushing through - if
problem persists, replace pedestal
complete assembly (20)
Connect inlet tube correctly
Free inlet valve by inserting a thin
rod into the inlet aperture
Unscrew the threaded platform
ring and make sure that the FEP 'O'
ring is properly fitted into its recess
if necessary stretch 'O' ring slightly
using fingertips
flush through with cleaning fluid
Clean unit by immersing platform
in cleaning fluid - if problem
persists, replace pedestal complete
assembly (20)
Calibrate the unit - See page 5
'User Calibration'
Clean platform base - if problem
persists, replace pedestal complete
assembly (20)
Unscrew the threaded platform
ring and make sure that the FEP 'O'
ring is properly fitted into its recess
if necessary stretch 'O' ring slightly
using fingertips
Replace FEP 'O' ring
2. Place the instrument into an empty sink together with its reservoir.
Unscrew the threaded platform base from the reservoir and lift the
dispenser's intake tube carefully out of the reservoir, whilst tapping
it against the reservoir's aperture to shake off any droplets from the
intake tube.
3. Hold the dispense tube nozzle over the aperture of the reservoir and
apply gentle piston strokes in order to return any glass barrel contents
into the reservoir.
4. Flush out remaining glass barrel contents with distilled water or a suitable
solvent. This will preserve the smooth action of the piston and free action
of the inlet and outlet valves. If the inlet valve does stick and is not
freed by flushing, it may be freed by gently inserting a thin rod into the
inlet aperture and gently pushing the ball off its seating. Re-check unit
for operation.
5. Empty the instrument completely after cleaning, and flush through with
distilled water.

Sterilising/Autoclaving

Autoclaving is permissible at 121 °C 2 bar after the normal cleaning
procedure is carried out. The cursor should be locked in the centre of the
adjustment slot before autoclaving.
Place the instrument on a cloth in order to avoid contact with metal surface.
Autoclave the unit in its assembled status but with the threaded ring
completely loosened. Autoclaving of the piston outside the glass barrel will
damage it. Cool slowly back to ambient temperature before use.
Chemical sterilisation
The fluid path components of the Zippette Classic can be soaked overnight
in a dilute (1:1000) solution of sodium hypochlorite. (See page 6 for
disassembly procedure).
Unit ready for
autoclaving with
threaded platform ring
completely loosened
Threaded ring
Anti-drip tap
ZIPPETTE CLASSIC
Cursor locked
in centre of
adjustment slot
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