Zuzi 4201/50 Manual De Instrucciones página 20

4.2 Quantitative mode
Zuzi spectrophotometer model 4201/50 allows performing quantitative analysis by the single point standard
curve method. The standard curve is obtained through a sample of known concentration. The absorbance of this
sample is measured and the equipment calculates the equation of the corresponding curve, which will be used for
the analysis of samples of unknown concentration.
Note: the curve will not be displayed but it will be used to measure the concentration of the samples.
Select Quantitative with F2 (2.6). First the system will ask to insert the blank (Fig. 13).
Figure 13
Insert the blank, the standard sample and the sample of unknown concentration into the sample compartment.
Put the blank into the light path.
Press GOTO λ (2.1) and adjust the working wavelength value.
Select OK with F1 (2.5) to make the blank.
Then the system will ask to insert the standard sample (Fig 14).
Put the standard sample into the light path and input the value of its concentration. For this, adjust the value digit
Figure 14
by digit with ▲▼ (2.7) (2.8); to go from one digit to the next one select OK with F1 (2.5). When the value of the last
digit is adjusted select OK with F1 (2.5) (Fig. 15).
Put again the blank into the light path and press ZERO (2.2) to make the blank.
Put the sample of unknown concentration into the light path and the concentration value will be displayed.
4.3 Deleting and printing results
At the result chart of any measuring mode, press PRINT (2.4) and the following options will be displayed (Fig.
16):
1. Print and clear: for printing and then, deleting data
2. Only clear, not print: for deleting data but not printing
3. Cancel
Select the desired option with ▲▼ (2.7) (2.8) and then confirm OK with F1 (2.5).
If you are not using the spectrophotometer for a long period of time please make sure it is disconnected from the
net and protected from dust (this way you will avoid accidents and will extend its working-life).
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Instruction manual 54201050 Revision 2 November-2010