Use Of The Microscope In Phase Contrast; Installing The Phase Contrast Slider; Phase Contrast Slider; Centering The Phase Ring - Optika Italy IM Serie Manual De Instrucciones

11. Use of the microscope in phase contrast

11.1 Installing the phase contrast slider

1. Insert the slider into the lower slot of the illumination system,
printed face up. (Fig. 24)
2. Pull the slider into the desired position, until it arrives to the
click stop.
3. When in phase contrast observation, keep the aperture dia-
phragm adjustment lever

11.2 Phase contrast slider

• The phase ring is pre-centered when the microscope leaves
the factory. It should therefore need no further adjustment.
Should a re-centering is needed, it can be performed via the
two side bolts (see chapter 10.3).
• The 4x/10x position
contrast objectives, the 20x/40x position
40x and the SL position
SLIDER
MEANING
POSITION
SL empty hole
4x/10x phase ring 4x/10x
20x/40x phase ring 20x/40x

11.3 Centering the phase ring

• Usually this operation is not needed. If necessary, please
proceed with the following steps:
1. Place a specimen on the stage and focus it.
2. Take out the eyepiece from the eyepiece tube without the di-
opter adjustment, and replace it with the centering telescope
(CT). (Fig. 26)
3. Check that the phase ring and the objective match, and that
both are steadily set on a click stop.
①
on the "O" (open) position.
②
must be used with 4x and 10x phase
③
④
is used for brightfield. (Fig. 25)
APPLICATION
brightfield
observation
phase contrast
observation with 4x and
10x objectives
phase contrast
observation with 20x
and 40x objectives
with the 20x and
Page 17
①
② ③
F ig. 24 F ig. 24
④
F ig. 25 F ig. 25
F ig. 26 F ig. 26