Problem
Cause
The pipette tip is
emptied too fast.
Droplets of
liquid remain in
The tip wettability
the pipette tip.
has increased due
to extensive use.
The pipette tip
immersion is too
shallow.
Droplets of air
The pipette tip is
incorrectly pressed
appear in the
liquid aspirated
onto the pipette
into the tip.
shaft.
The tip is damaged
or worn out due to
extensive use.
The pipette tip is
incorrectly pressed
onto the pipette
shaft.
The shaft nut is
loose (Fig. 3F)
in the models
2 - 1000
The pipette
incorrectly
aspirates the
liquid or liquid
drops out from
the tip.
The sealing surface
of the shaft is
cracked or scored.
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Solution
Decrease the speed of press-
ing the pipette pushbutton.
Replace the tip with a new
one.
Immerse the tip deeper
according to the instructions.
Press the pipette tip firmly.
Replace the tip with a new
one.
Press the pipette tip firmly.
Tighten the shaft nut.
Remove the tip ejector.
Unscrew the shaft nut, inspect
the shaft and the piston as-
sembly. Replace the damaged
parts (see Section 12). When
reasembling the pipette, the
nut should be hand tightened.
In the models AP2, AP10 and
AP20, the damage of the shaft
may also cause a damage of
the piston assembly. Replace
the damaged parts (see Sec-
tion 12). When reasembling
the pipette, the nut should be
hand tightened.To remove the
tip ejector in models AP5000
and AP10000, remove the
ejector pushbutton (Fig. 3N)
and using a screwdriver
unscrew the tip ejector by
turning the screwdriver
counter-clockwise.
Problem
Cause
Damage to the
piston or seal due
to prolonged use
with the aggressive
liquids.
The pipette
is reassembled
The pipette
improperly.
incorrectly
aspirates the
liquid or liquid
drops out from
the tip.
No lubricant on the
sealing elements.
Contamination of
the inside of the
pipette caused
by extensive
aspiration of chemi-
cally aggressive
liquids or because
liquid got inside the
pipette.
If you find an increase in the pipetting force, which could
happen after repetitive autoclaving of the pipette:
Remove the ejector. Unscrew the shaft nut, and then remove
the shaft, piston assembly, seal and O-ring. Rinse the removed
parts in distilled water and dry. Lubricate the internal surfaces
of the seal and O-ring with lubricant that has been included
with each pipette. Reassemble the pipette in opposite order.
Note: All parts of the pipette can be autoclaved (see Section
10). The shaft of the AP5000 and AP10000 models should
be autoclaved without the filter.
ENGLISH
Solution
Disassemble the pipette as
described above. Replace the
piston, seal and O-ring (see
Section 12). Rinse the inside
of the shaft in distilled water
and dry. Lubricate the seal and
O-ring with the lubricant, that
is included with each pipette.
Following piston replacement,
a calibration procedure is
required.
Note: The parts of AP2 and
AP10 pipette should be lubri-
cated evently with a minimum
amount of lubricant.
Disassemble the pipette and
reassemble it, observing the
proper sequence of steps
(Fig. 3).
Remove the tip ejector
Unscrew the shaft nut, remove
the shaft, piston assembly,
seal and O-ring. Rinse the re-
moved parts in distilled water
and dry thoroughly. Lightly
lubricate the inside surfaces
of the seal and the O-ring
with the included lubricant.
Reassemble the pipette in the
reverse order.
Remove the tip ejector.
Unscrew the nut, remove the
shaft, piston assembly, seal
and O-ring. Rinse the removed
parts with distilled water
and dry thoroughly. Lightly
lubricate the inside surfaces
of the seal and the O-ring with
the lubricant. Reassemble the
pipette in the reverse order.
19