Interpretation Of Results; Disposal; Audible Alarm; Cancellation Of A Reading - TerraTec Bionova MiniBio Manual Del Usuario

Instructions for use
Read the instructions for use of each SCBI for
more information.
Press the cap to seal the tube and crush the ampoule, make sure
the media completely wets the carrier. Write a C (for control) and
the date on the label. The positive control can be placed in any
position of the Bionova MiniBio incubator. The positive control
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should belong to the same batch of the processed SCBI.
It is a good practice to use a positive control, this helps ensuring:
•Correct temperature is reached.
•Viability of spores has not been altered due to improper storage
temperature, humidity, or proximity to chemicals.
•Capability of the media to promote rapid growth and
fluorescence generation.
•Proper functioning of Bionova

Interpretation of results

Rapid Readout
The indicator used as positive control should show a positive
result (red light ). The processed SCBI results are not
valid until the biological indicator used as positive control
shows a positive result
positive result, indicates a failure in the sterilization process. A
negative result, (green light
min. (60 ºC), 1 h. (60 ºC), 2 hs. (60 °C), 3 hs. (60 ºC) 4 hs. (37 ºC)
according to the used program, indicates that the sterilization
process was effective.
Take action on any positive result immediately. Always retest
the sterilizer and do not use the sterilizer for processing loads
until 3 consecutive SCBI results are negative.
Visual Readout
The Rapid readout system allows the confirmation of results by
color change after 48 hours or 7 days incubation analysis (if 7-day
readout is made, an humidified incubator will be required to avoid
media dry out). If the sterilization process was not successful,
the growth media contained in the indicator will turn to yellow,
as it is shown in Instruction of Use of SCBIs. If the sterilization
process was successful, the media will remain unchanged.
MiniBio incubator.
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. A processed SCBI with a
) after 20 min. (60 ºC), 30
Bionova MiniBio Page 10
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The color of the growth media in the SCBI used
as positive control must turn to yellow during
the process of incubation for the results to be
valid. Record the positive ones and discard them
immediately as shown in the biological indicators
instructions for use.

Disposal

Dispose SCBls after use, according to your healthcare and
safety regulations. Positives SCBIs can be sterilized (see SCBIs
instructions for use). It is not possible to reuse a SCBIs.

Audible alarm

An audible alarm will sound every time a positive result is
detected in the Bionova MiniBio incubator. The alarm allows
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the user to immediately detect a positive result without the
need to visually control the equipment. The alarm can be
cancelled by pressing the button

Cancellation of a reading

When a SCBI is removed from its position during incubation
process, an audible alarm will sound to indicate that the tube
should be returned to the corresponding position.
If the indicator is not re-introduced into the reading position
after 10 seconds of being withdrawn, the reading will be
cancelled and the cancelation will be informed through the
printing of a ticket.

Temperature monitoring

The Bionova MiniBio incubator contains an internal
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temperature control. If the temperature falls outside of the
specified range (37±2) ºC or (60±2) ºC, the blue temperature
led light will start to twinkle.
Temperature can be externally monitored by using a Bionova
TB-IC1020 thermometer or other similar. The Auto-Reader
MiniBio has a special hole for placing the thermometer which is
located at the right side of the device.
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