IM-3FL4
Move the filter lever (placed on the left side of the
microscope) ④ to insert the desired filter cube: B, G
(V and UV - optional). (Fig. 49)
IM-3F / IM-3LD
FILTER
EXCITATION
CUBE
FILTER
B
460-490 nm
G
540-580 nm
IM-3FL4
FILTER
EXCITATION
CUBE
FILTER
B
460-490 nm
G
527-553 nm
UV
325-375 nm
V
390-420 nm
Only for IM-3LD
Use the anti-glow cap to prevent flare coming from
the condenser front lens. (Fig. 50)
DICHROIC
EMISSION
MIRROR
FILTER
495 nm
520LP nm
585 nm
590LP nm
DICHROIC
EMISSION
MIRROR
FILTER
500 nm
520LP nm
565 nm
575LP nm
415
435LP nm
440 nm
455 LP nm
④
•
FITC: fluorescent antibodies
•
Acridine orange: DNA, RNA
•
Auramine
•
Rhodamine, TRITC: fluorescent antibodies
•
Propidium iodide: DNA, RNA
•
RFP
•
FITC: fluorescent antibodies
•
Acridine orange: DNA, RNA
•
Auramine
•
Rhodamine, TRITC: fluorescent antibodies
•
Propidium iodide: DNA, RNA
•
RFP
•
Nuclear counterstaining
•
Acridine orange: DNA, RNA
Page 26
APPLICATIONS
APPLICATIONS
Fig. 49
Fig. 50