Thermo Finnpipette 300 Instrucciones De Uso página 7

DEVICE REQUIREMENTS AND TEST CONDITIONS
An analytical balance must be used. The scale graduation value of the balance should be
chosen according to the selected test volume of the pipette:
Volume range
under 10µl
10-100µl
above 100µl
Test liquid: Water, distilled or deionized, "grade 3" water conforming ISO 3696. Tests are
done in a draft-free room at a constant (±0.5°C) temperature of water, pipette and air
between 15°C to 30°C. The relative humidity must be above 50%. Especially with
volumes under 50µl the air humidity should be as high as possible to reduce the effect of
evaporation loss. Special accessories, such as the evaporation trap, are recommened.
CHECKING THE CALIBRATION
The pipette is checked at the maximum volume (nominal volume) and the minumum
volume or 10 % of the maximum volume, whichever is higher. E.g. Finnpipette 0.5-10 µl
is tested at 10 µl and 1 µl. Both volumes are tested with the two end channels. A new tip
is first pre-wetted 3-5 times and a series of ten pipettings are done with both volumes. A
pipette is always adjusted for delivery (Ex) of the selected volume. Measuring volumes
taken from balance is not allowed. If the calculated results are in the limits, the calibration
of the pipette is correct.
Range Channel Volume
0,5-10 µl 8
5-50 µl 8
50-300 µl 8
0,5-10 µl 12
5-50 µl 12
50-300 µl 12
5-50 µl 16
ADJUSTMENT:
Adjustment is done with the service tool.
1. The adjustment is done at the lower volume with one of the middle channels.
2. Place the service tool into the openings of the calibration nut at the top of the handle.
3. Turn the service tool clockwise to increase, or counterclockwise to decrease the volume.
4. After adjustment check the calibration according to the instructions above.
readable graduation
0.001mg
0.01mg
0.1mg
Accuracy
µl µl %
10 ±0.24 ±2.4
1 ±0.12 ±12.0
50 ±0.75 ±1.5
5 ±0.25 ±5.0
300 ±3.0 ±1.0
50 ±2.3 ±4.6
10 ±0.24 ±2.4
1 ±0.12 ±12.0
50 ±0.75 ±1.5
5 ±0.25 ±5.0
300 ±3.0 ±1.0
50 ±2.3 ±4.6
50 ±0.75 ±1.5
5 ±0.25 ±5.0
Procedure:
Precision 1. Do 10 pipettings with the
s.d. µl cv%
minimum volume.
2. Do 10 pipettings with the
0.16 1.6
0.08 8.0 maximum volume.
0.35 0.7
3. Calculate the accuracy
0.10 2.0
(A) and precision (cv) of
0.9 0.3
both series.
0.8 1.5
4. Compare the results to the
0.16 1.6
limits in the Table 1.
0.08 8.0
If the results are in the limits of
0.35 0.7
Table 1, then the calibration of
0.10 2.0
the pipette is correct.
0.9 0.3
0.8 1.5
Otherwise the pipette must be
0.35 0.7
adjusted and checked again.
0.10 2.0
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