General Use; Using The Microscope - Levenhuk 320 Serie Guia Del Usuario

Levenhuk 320 Series Biological Microscopes
Never, under any circumstances, look directly at the Sun, another bright source of light or
at a laser through this device, as this may cause PERMANENT RETINAL DAMAGE and may lead
to BLINDNESS.

General use

Levenhuk 320 series biological microscopes are designed for observations of specimens in the
transmitted light. They are used in many scientific fields, including biology, ecology, medicine and
veterinary science, as well as for technological research and school presentations.
Microscope parts
Base. It houses the illumination source, electronics, and control mechanisms.
Arm. This piece holds the base, the stage and the head of the microscope together.
Rack-and-pinion mechanism. The stage with the condenser are mounted on the arm and are
moving vertically along it. Coarse and fine focus systems provide for smooth vertical movements of
the stage. For additional precision, a condenser may be adjusted separately.
Head. A monocular head is mounted at a 45° angle at the upper end of the arm and is 360°
rotatable.
Eyepieces and objectives. Consist of lenses that allow magnifying the image. High-quality
achromatic objectives with 4x, 10x, 40x and 100x magnifications provide for sharp and bright
images. The total magnification is calculated by multiplying the eyepiece magnification to the
objective magnification.
Revolving nosepiece. The quadruple revolving nosepiece allows you to change objectives
smoothly and easily.
Stage. Sturdy and reliable stage with a specimen holder and mechanical scale can be used to move
your slides while observing them. The lower illumination light passes through the opening in the
middle of the stage.
Condenser. Abbe condenser, 1.25 N.A. iris diaphragm is a system of lenses located below the stage
that allows gathering and directing the light rays.
Focusing knob. A coarse and fine focusing system allows moving the stage up and down adjusting
the image sharpness.
Lower illumination. Illumination with adjustable brightness allows observing transparent objects.
The illumination system can be powered by batteries or an AC power supply.

Using the microscope

Getting started
• Unpack the microscope and make sure all parts are available.
• Move the stage to the lowermost position using the focusing knob.
• Connect the microscope to AC power using the AC adapter. Levenhuk 320 PLUS microscopes
can be operated in standalone mode. To do this, you need to charge the batteries included in
the kit.
• Turn on the illumination or adjust the reflector to evenly illuminate the specimen within the
field of view.
• Insert the eyepiece into the eyepiece tube.
• Slowly adjust the illumination brightness, from dark to light. When you finish your
observations, always dim the illumination before unplugging the power cable.
• If you need to change the light bulb, unplug your microscope first.
Focusing
• Place a specimen on the stage and fix it with the holders.
• Select the 4x objective rotating the revolving nosepiece.
• Center the observed specimen in the field of view.
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