Pipetting Techniques - Thermo Finnpipette BioControl Instrucciones De Uso

STEPPER:
After selecting and confirming
volume using the + and - keys. The unit volume figures will blink until
you confirm your choice by pressing SET. Once the unit volume is set,
the number of repetitions will start to blink on the display. The first option
is the maximum number of repetitions. Use the + and - keys to choose
the appropriate volume. Press
The maximum number of repetitions depends on the size of the tip cone
module and the adjusted unit volume. If the maximum number of
repetitions is needed, press
(Fig. 9).
PISTON SPEED
Before pipetting, select the piston speed by adjusting the switch on top
of the pipette, (Fig. 4). The three pipetting speeds are fast, normal and
slow. Fast pipetting is for aspirating and dispensing liquid solutions with
a viscosity and surface tension similar to water.
Slower speeds are required for liquids with a high viscosity, low surface
tension or a tendency to foam.

PIPETTING TECHNIQUES

Figures 10-12: A = Ready position
B = First stop
C = Second stop
FORWARD AND REVERSE PIPETTING IN PIPETTE MODE
In PIPETTE mode, forward and reverse pipetting are possible without further programming. The two stops of the
pipetting trigger correspond to the position of the piston, just as in manual pipetting.
FORWARD PIPETTING, (Fig.10)
1. Press the trigger to the first stop.
2. Hold the pipette in a vertical position and dip the tip ends in the liquid. Let the trigger return to the
ready position. Withdraw the tips from the liquid, touching them against the edge of the reservoir to
remove excess liquid.
3. Place the tips against the wall of the receiving vessel. Dispense the liquid by pressing the pipetting
trigger to the second stop. The dispense blow-out action will empty the tips. Remove the tips from the
liquid, sliding them along the wall of the vessel.
4. Release the trigger to the ready position.
If necessary, change the pipette tips and continue pipetting.
REVERSE PIPETTING, (Fig.11)
The reverse technique is suitable for dispensing liquids with a high viscosity or tendency to foam. The
technique is also recommended for dispensing very small volumes.
1. Press the pipetting trigger to the second stop.
2. Holding the pipette in the vertical position, dip the tip ends in the liquid and release the trigger. This
action will fill the tips. Remove the tips from the liquid, touching them against the reservoir to remove
excess liquid.
3. Dispense the preset volume by gently depressing the trigger to the first stop.
Hold the pipetting trigger at the first stop. The liquid remaining in the tips should not be included in the
delivery.
4. The remaining liquid should be discarded by pressing the trigger to the second stop, or aspirated with
the next pipetting sequence.
STEPPER mode, (Fig.8), set the
SET to confirm your choice.
SET when MAX STEP appears on screen
STEP
STEPPER
SET
Fig.8. Display in STEPPER mode
MAX
STEP
STEPPER
SET
Fig.9. Display in STEPPER mode
5
4 25
x
µl
-
+
MODE
I2 25
x
µl
-
+
MODE