Calibration; General Information - StarLab ErgoOne Manual De Usuario

6. Calibration

During manufacture, ErgoOne® pipettes are aligned for transferring
aqueous solutions and calibrated according to EN ISO 8655. We there-
fore also recommend that you regularly check your pipettes at defined
intervals of, for example, 3 or 12 months.
Generally, realignment is not necessary with normal use. However,
if your pipettes still do not achieve the specified limiting values
(see section 5 Technical Data), check the following points:
• Is the pipette seal broken?
Is it dripping? (see section 3 Troubleshooting)
• Is the density of the liquid you are using different from that of water?
• Are there any temperature differences between the pipette, liquid
and air?
• Was the liquid pipetted slowly and evenly?
If the check is unsuccessful, calibrate and realign your pipette. This
can be carried out according to the following instructions. Alternatively,
STARLAB offers a professional Pipette Calibration Service with a
fast turnaround.
Note
that each alignment means a zero point shifting. The amount
by which the pipette setting is changed is constant across the entire
measurement range. If for example 1000µl is realigned by 10µl
(1%) for a 100 –1000µl pipette, then for 100µl, the pipette must
also be corrected by 10µl = 10%.
Calibration & alignment
Calibration is a gravimetric analysis for which you require an analytical
balance adjusted to the pipetting volume, a weighing vessel and
distilled water (room temperature).

General information

• Only use TipOne
• Same temperature for pipette, tip and water
• Each tip must be pre-wetted with distilled water at least three times.
• Always pipette evenly and slowly and hold the pipette upright.
• Immerse no more than 3 mm of
the pipette tip into the liquid when taking a sample.
• Position the pipette at an angle of 30 – 45° to the inner wall of the
tube when dispensing liquid.
• For multi-channel pipettes, all channels must be checked separately.
pipette tips and attach these securely.
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ErgoOne User Manual
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