Leica CV5030 Manual De Instrucciones página 44

Media Parameter Settings
Mountant Brand
Based on Toluene:
Mounting-Medium
Toluene
Acrylmount Toluene
Acrytol
Mounting Medium
Toluene
Cytoseal 280
Mounting Medium
Toluene
Mounting Medium T T T T T
Toluene
Protocol Secure Mount Fisher Health Care
Mounting Medium
Toluene
Protocol
Mounting Medium
Toluene
Not recommended:
Medium
Neo-Mount:
(tested with Neo-Clear/Merck)
Roti-R-Histokitt
(tested with Rotihistol/Roth)
Volume settings were defined under the following conditions: wet coverslipping, temperature approx. 25 °C, histology sections of
approx. 3µm thickness, 50 mm coverslip, Stroke: 50. Optimized volume settings depend on and are affected by various factors.
Factor
Dry coverslipping
High temperature (> 28°C)
Low temperatures (< 22°C)
Monolayer sample type
44
Manufacturer
Richard Allan Scientific
Anapath Stat Lab
Surgipath
Stephens Scientific
Acra Lab
Fisher Scientific Company
Because of
Very low viscosity:
May result in excess medium
Inconsistent stroke
Effect on volume setting
+
-
+
-
Type Volume Nozzle
5 2 21
4 4 21
4 7 21
5 8 21
1 2 21
or
1 1 21
5 3 20
1 1 21
But best result with:
- Type: 5
- Volume: 1
Factor
Smear sample type
Coverslip size 40 mm
Coverslip size 60 mm
Manual de instrucciones V 1.2 – 11/2002
Comment
Correction:
Length: -50
Correction:
Length: -50
Correction:
Length: -100
Correction:
Length: -50
Correction:
Length: -50
STP: -50
Correction:
Length: -50
- Nozzle: 21
- Length: -100
Effect on volume setting
+
- or shorter stroke type
++ or longer stroke type