Troubleshooting; Quality Control; Technical Assistance - INGENASA INgene q IBV SYBR Instrucciones De Uso

10. TROUBLESHOOTING

Detection of fluorescence signal on SYBR channel in the negative extraction control.
Possible cause
Contamination during extraction
process
Detection of fluorescence signal on SYBR channel (pathogen) in the negative PCR control.
Possible cause
Contamination during RT-PCR
preparation.
Absence of fluorescence signal on SYBR channel in the positive PCR controls.
Possible cause
Wrong fluorochrome channel
selected

11. QUALITY CONTROL

Each production batch of this kit has been checked under our ISO-certified Quality Management System.

12. TECHNICAL ASSISTANCE

For further information concerning the assay and its performance, please contact:
INSTRUCTIONS – ENGLISH
Solution
Repeat the RNA extraction process and RT-PCR using new reagents.

Ensure that the workplace, equipment and all instruments used for

performing the RT-PCR are decontaminated, i.e. free from nucleic
acids.
Solution
Repeat RT-PCR reaction with new reagents and, if possible, various

replicates of each sample.
If tubes are being used, close each one immediately after adding

the sample.
As a strict rule, the positive control must be added last and in a

separate physical location from where the problem samples are
added.
Ensure that the workplace, equipment and all instruments used for

performing the RT-PCR are decontaminated, i.e. free of nucleic
acids.
Solution
Check that you select the SYBR channel for all samples and controls

to be analyzed.
INGENASA
C/ Hnos. García Noblejas, 41 – 28037 MADRID
Tel (+34) 91 368 0501
e-mail: [email protected]
(Versión 1410-14) – PAG.12.