3M L25PNT Instrucciones Del Producto página 5

3. Liquid Samples Except Liquid Chocolate Samples
3.1 Label one microcentrifuge tube for each Liquid sample.
3.2 Measure 900 μL of 3M Extraction Buffer into a labeled microcentrifuge tube.
3.3 Add 100 μL of a well-mixed sample. Shake vigorously or vortex for 15 seconds to mix thoroughly to obtain an
extracted sample.
Note: The pH of extracted sample should be between 5 and 10. Proceed to Troubleshooting section for
additional information.
3.4 Remove one 3M Peanut Protein LFD from package and place on a clean, dry, flat surface.
3.5 Transfer 100 μL of the extracted sample prepared in 3.3 from the middle (aqueous) layer using a clean pipette
or pipette tip and apply it to the sample well on the 3M Peanut Protein LFD. Start the timer for 11 ± 1 minutes.
Proceed to Interpretation of Results section.
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